急性毛细支气管炎患儿外周血单个核细胞中SIRT1水平表达与预后的相关性研究

目的 分析急性毛细支气管炎患儿外周血单个核细胞中沉默信息调节因子1(silent information regulator 1,SIRT1)水平表达与预后的相关性。方法 选择2019年10月～2021年1月在唐山市妇幼保健院就诊的156例急性毛细支气管炎患儿作为研究对象，并根据出院后6个月是否发生喘息分为喘息组（n=51）和非喘息组（n=105）。用Ficoll密度梯度离心法分离外周血中单个核细胞，用蛋白质免疫印迹法检测单个核细胞中SIRT1水平。比较喘息组和非喘息组治疗前后SIRT1水平，并分析其与急性支气管炎治疗后喘息的关系。结果 喘息组的过敏史占比高于非喘息组[25.49%(13/51) vs 6.67%(7/105)]，差异有统计学意义（χ2=10.882,P=0.001）。喘息组治疗前、治疗后的SIRT1水平均低于非喘息组（0.13±0.03 vs 0.18±0.05,0.31±0.06 vs 0.37±0.08），差异均有统计学意义（t=7.703,5.814，均P=0.000）。治疗前SIRT1判断急性毛细支气管炎患儿治疗后发生喘息的受试者工作特征（ROC）曲线下面积为...

Correlation between the Expression of SIRT1 in Peripheral Blood Mononuclear Cells and Prognosis in Children with Acute Capillary Bronchitis

Objective　To analyze the correlation between the expression of silent information regulator 1(SIRT1) in peripheral blood mononuclear cells and prognosis in children with acute capillary bronchitis. Methods　156 children with acute capillary bronchitis attending the Tangshan Maternal and Child Health Hospital from October 2019 to January 2021 were selected for the study and divided into a wheezing group (n=51) and a non-wheezing group (n=105) based on whether wheezing occurred in 6 months after discharge from hospital. Mononuclear cells in peripheral blood were isolated by FICOLL density gradient centrifugation, and SIRT1 in mononuclear cells were detected by Western blotting. The pre-treatment and post-treatment SIRT1 levels in the wheezing group and the non-wheezing group were compared and their relationship with wheezing after treatment of acute capillary bronchitis was analysed. Results　The percentage of allergy history was higher in the wheezing group than in the non-wheezing group [25.49%(13/51) vs. 6.67%(7/105)],the difference was statistically significant（χ2=10.882, P=0.001). Pretreatment and post-treatment SIRT1 levels were lower in the wheezing group than in the non-wheezing group (0.13±0.03 vs 0.18±0.05, 0.31±0.06 vs 0.37±0.08）, the differences were statistically significant（t=7.703，5.814, all P =0.000). The area under the receiver operating characteristics (ROC) curve of pre-treatment SIRT1 for judging wheezing in children with acute capillary bronchitis after treatment was 0.814, which was higher than that of post-treatment SIRT1 (0.734）, the difference was not statistically significant（Z =1.498, P=0.134). A history of allergy was an independent risk factor for the development of wheezing after treatment in children with acute capillary bronchitis (OR=3.864, P =0.017), and a pre-treatment SIRT1 ＞ 0.15 was an independent protective factor for the development of wheezing after treatment in children with acute capillary bronchitis (OR=0.120, P =0.000). Pre-treatment SIRT1 was linearly associated with the development of wheezing after treatment for acute capillary bronchitis (non-linear test χ2=2.36, P =0.125). Conclusion　The low level of pre-treatment SIRT1 in peripheral blood mononuclear cells was associated with wheezing in children with acute capillary bronchitis after treatment.