大鼠牙齿移动过程中Piezo1对压力侧牙周组织中RIP3及MLKL的影响

目的 探究大鼠牙移动过程中，在不同时间点Piezo1对压力侧牙周组织中坏死性凋亡标志物受体相互作用蛋白3(receptor-interacting protein 3,RIP3)、混合谱系激酶结构域样蛋白(mixed lineage kinase domain-like protein, MLKL)的影响。方法 选用雄性SD大鼠75只，随机分为对照组(A组),加力组(B组),加力+抑制剂组(C组)。构建大鼠上颌第一磨牙移动的正畸模型，通过游标卡尺测量正畸牙向近中移动的距离，应用苏木精-伊红(hematoxylin-eosin, HE)染色观察压力侧牙周组织变化，免疫组化法检测RIP3、MLKL表达量的变化。结果 与A组相比较，B组和C组牙周组织中RIP3、MLKL表达均呈先增加后下降的趋势(P<0.05),其中RIP3表达量增加至5 d时达到高峰，MLKL表达量增加至3 d时达到高峰；与B组相比较，C组牙周组织中RIP3、MLKL表达整体下降(P<0.05),表达趋势变化与B组一致。结论 Piezo1通道在正畸牙齿移动过程中可能起着机械转导的作用，阻断Piezo1通道可下调...

Effect of Piezo1 on RIP3 and MLKL in periodontal tissue of the pressure side during tooth movement in rats

Objective To observe the influence of Piezo1 on the necroptosis markers receptor-interacting protein 3 (RIP3) and mixed lineage kinase domain-like protein (MLKL) in pressure-side periodontal tissue at various time points during tooth movement in rats. Methods Seventy-five male SD rats were randomly distributed into control group (group A), force group (group B) and force + inhibitor group (group C). The orthodontic model of the maxillary first molar was constructed in rats. The moving distance of the orthodontic tooth was measured with vernier calipers. Hematoxylin-eosin (HE) staining was used to observe changes of pressure-side periodontal tissue, and changes of RIP3 and MLKL were tested by immunohistochemical staining. Results Compared with group A, expression levels of RIP3 and MLKL in periodontal tissue in group B and C increased firstly and then fell (P<0.05), and the expression of RIP3 increased to the peak on the 5th day, MLKL on the 3rd day. Compared with group B, the expression of RIP3 and MLKL in periodontal tissue in group C were decreased as a whole (P<0.05), and the expression trend was consistent with group B. Conclusion Piezo1 channel may play a role in the mechanotransduction process of orthodontic tooth movement. Blocking the function of Piezo1 channel may reduce the expression of RIP3 and MLKL in pressure-side periodontal tissue, reduce the occurrence of necroptosis and the speed of orthodontic tooth movement.