miR-127-3p对口腔鳞状细胞癌细胞增殖、迁移及Zwint-1基因表达的影响

目的 探讨miR-127-3p调控Zeste white 10(ZW10)相互作用着丝粒蛋白1(Zwint-1)对口腔鳞状细胞癌(OSCC)细胞增殖、迁移及侵袭的影响.方法 体外培养OSCC细胞系PE/CA-PJ15、CAL27,对其转染并分为空白对照组(NG组)、阴性转染组(NC组)、过表达miR-127-3p组(m...

Effects of miR-127-3p on proliferation,migration and Zwint-1 gene expression of oral squamous cell carcinoma cells

Objective To investigate the effect of miR-127-3p on proliferation, migration and invasion of oral squamous cell carcinoma (OSCC) cells by regulating Zeste white 10 (ZW10)-interacting kinetochore protein 1 (Zwint-1). Methods PE/CA-PJ15, CAL27 cells were cultured in vitro, transfected and divided into blank control group (NG group), negative transfection group (NC group), miR-127-3p overexpression group (miR-127-3p-mimics group). The transfection effect was observed under inverted fluorescence microscope; levels of miR-127-3p and Zwint-1 mRNA were detected by real-time PCR; cell proliferation was detected by MTT assay; the migration ability and invasion ability of PE/CA-PJ15 and CAL27 cells were detected by scratch test and Transwell assay respectively; expressions of human proliferating cell nuclear antigen (Ki-67), Apoptosis-associated protein Bax, Bcl-2, E-cadherin, N-cadherin, Vimentin and Zwint-1 were detected by Western blotting; the targeting relationship between miR-127-3p and Zwint-1 was predicted by TargetScan database and verified by double luciferase reporter gene experiment. Results Cells were successfully transfected; compared with those in NG group and NC group, the expression of miR-127-3p, proliferation inhibition rate and E-cadherin, Bax in PE/CA-PJ15 and CAL27 cells in miR-127-3p-mimics group were significantly increased (P<0.05); the wound healing rate, the number of cell invasion, the expression of Zwint-1 mRNA and protein, Ki-67, Bcl-2, N-cadherin and Vimentin protein were significantly decreased (P<0.05). TargetScan database prediction showed that Zwint-1 was a potential target gene of miR-127-3p; double luciferase reporter gene experiment confirmed that there was a targeting relationship between Zwint-1 and miR-127-3p, and miR-127-3p could negatively regulate the expression of Zwint-1. Conclusion Up-regulating the expression of miR-127-3p can target and down-regulate the expression of Zwint-1, and inhibit the proliferation, invasion and migration of OSCC cells.