Claudin-7在口腔鳞状细胞癌中的表达和作用研究

目的 探究Claudin-7在口腔鳞状细胞癌(OSCC)中的表达及作用。方法 通过免疫组化实验和蛋白质免疫印记实验检测OSCC中Claudin-7的表达情况，并分析Claudin-7和不同病理参数的关系。通过慢病毒转染Cal27和HN6细胞系上调Claudin-7表达，通过CCK-8和Transwell实验检测细胞增殖及迁移能力的变化；通过蛋白质免疫印迹实验和实时荧光定量聚合酶链式反应(RT-qPCR)检测Claudin-7对上皮间充质转化(EMT)指标的影响。结果 Claudin-7在OSCC中低表达(P<0.05),其表达水平和OSCC患者肿瘤大小、分化程度和淋巴结转移相关(P<0.05)。过表达Claudin-7可抑制Cal27和HN6细胞增殖和迁移，并且上调E-钙黏蛋白(E-cadherin)及下调N-钙黏蛋白(N-cadherin)、波形蛋白(Vimentin)的蛋白和mRNA(P<0.05)。结论 Claudin-7在OSCC中低表达，上调其表达可通过影响上皮间充质转化抑制OSCC细胞的增殖和迁移。Claudin-7可作为OSCC治疗的一个新靶点。

Expression and role of Claudin-7 in oral squamous cell carcinoma

Objective To explore the expression and role of Claudin-7 in oral squamous cell carcinoma (OSCC). Methods Expression of Claudin-7 in OSCC was detected by immunohistochemistry experiment and western blot experiment, and the relationship between Claudin-7 and pathological parameters was analyzed. Lentiviral was used to transfect Cal27 and HN6 cell line to increase the expression of Claudin-7. CCK-8 assay and Transwell assay were used to detect changes in cell proliferation and migration ability. Western blot experiments and RT-qPCR experiments were used to detect the effect of up-regulated Claudin-7 on the index of epithelial-mesenchymal transition. Results Claudin-7 was decreased in OSCC (P<0.05) and its expression level was related to patient′s tumor size, differentiation degree and lymph node metastasis (P<0.05). Overexpression of Claudin-7 could inhibit the proliferation and migration of Cal27 and HN6 cells, up-regulate the expression of E-cadherin protein and mRNA, and down-regulate the expression of N-cadherin and Vimentin protein and mRNA (P<0.05). Conclusion Claudin-7 is expressed at low levels in OSCC. Up-regulating the expression of Claudin-7 can inhibit the proliferation and migration of OSCC cells by regulating epithelial-mesenchymal transition. Claudin-7 can be used as a new target for the treatment of OSCC.