Monoclonal antibodies to monkey brain choline acetyltransferase: production and immunohistochemistry |
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| Authors: | T Ichikawa I Ishida Y Hirata T Deguchi |
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| Affiliation: | Department of Anatomy and Embryology, Tokyo Metropolitan Institute for Neurosciences, Japan. |
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| Abstract: | Two monoclonal antibodies to monkey brain choline acetyltransferase (ChAT) were obtained. Immunoblot analysis indicated that both antibodies revealed two adjacent protein bands on a sodium dodecyl sulfate-polyacrylamide gel electrophoresis. ChAT was demonstrated immunohistochemically by one of the antibodies in the motoneurons in the monkey brainstem and spinal cord. This antibody also revealed ChAT-positive terminal-like structures in the neuropils of lamina IX of the cervical spinal cord and interpeduncular nucleus. |
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