猪皮肤成纤维细胞的培养与鉴定

目的　研究猪内源性逆转录病毒在体内和体外的感染性 ,建立理想的细胞模型 ,为猪一人间跨种移植的生物安全性评价奠定良好的基础。方法　运用组织块培养法、冷胰蛋白酶方法及热胰蛋白酶方法培养猪皮肤成纤维细胞 ,比较 3种方法的优点和缺点。结果　建立了猪皮肤成纤维细胞系组织块培养法优于胰酶法。结论　应用组织块培养法培养猪皮肤成纤维细胞优于胰酶法 ,经济实惠、操作简便、易于掌握 ;并为研究PERV在体内和体外的感染性提供了理想的细胞模型 ,为评估猪一人间跨种移植的生物安全性提供一种新的方法 ;对建立动物克隆生产的皮肤体外培养模式具有重要的参考价值。

Cultivation and identification of porcine skin fibroblasts

Objectives To establish an ideal cellular model for the study of infection of porcine endogenous retrovirus in intro and in vivo and to lay the foundation for evaluating the biological safety of xenotransplantation. Methods Porcine skin fibroblasts were cultured by tissue pieces, cold trypsin, and heat trypsin, respectively. The merit and defect of these methods were analyzed. Results Porcine skin fibroblast line was established and the tissue piece method was superior to trypsin methods. Conclusion Culture of porcine skin fibroblasts with tissue pieces is superior to trypsin method, with the characteristics of low cost and easy operation. The established porcine skin fibroblast cell line supplies an ideal cell model for the study of infection of porcine endogenous retrovirus in vitro and in vivo, provides a new method for the evaluation of biological safety of xenotransplantation, and plays an important role in the skin culture model of production of cloned animals.