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| 海康灵含药血清在体外对SH-SY5Y神经细胞损伤的保护作用 | |
| 引用本文： | 孙向红,刘洪玲,耿美玉.海康灵含药血清在体外对SH-SY5Y神经细胞损伤的保护作用[J].中国药学杂志,2006,41(19):1465-1467. |
| 作者姓名： | 孙向红 刘洪玲 耿美玉 |
| 作者单位： | 1. 中国海洋大学海洋药物与食品研究所,山东,青岛,266003;青岛大学医学院附属医院,山东,青岛266003 2. 青岛大学医学院附属医院,山东,青岛266003 3. 中国海洋大学海洋药物与食品研究所,山东,青岛,266003 |
| 摘 要： | 目的观察海康灵含药血清(HKL-serum)对SH-SY5Y神经细胞损伤的保护作用。方法采用体外细胞培养的方法,建立SH-SY5Y神经细胞过氧化氢(H2O2)损伤模型。通过观察细胞形态,测定细胞存活率(MTT法)、乳酸脱氢酶(LDH)漏出率,检测HKL-serum对SH-SY5Y神经细胞损伤的保护作用。结果同造模组比较,10%,15%HKL-serum能减轻H2O2引起的SH-SY5Y神经细胞的损伤,明显提高细胞的存活率,减少LDH的释放量。结论结果显示,HKL-serum对SH-SY5Y神经细胞损伤具有明显的保护作用。 |
| 关 键 词： | 海康灵 H2O2 SH-SY5Y神经细胞 |
| 文章编号： | 1001-2494（2006）19-1465-04 |
| 收稿时间： | 2005-08-08 |
| 修稿时间： | 2005-08-08 |
Protective Effects of Haikangling on Neuroblastoma SH-SY5Y Cells in vitro |
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| SUN Xiang-hong,LIU Hong-ling,GENG Mei-yu.Protective Effects of Haikangling on Neuroblastoma SH-SY5Y Cells in vitro[J].Chinese Pharmaceutical Journal,2006,41(19):1465-1467. | |
| Authors: | SUN Xiang-hong LIU Hong-ling GENG Mei-yu |
| Institution: | 1. Marine Drug and Food Institute, Ocean University of China, Qingdao 266003, China ; 2. Hospital Affiliated to Medical College, Qingdao University, Qingdao 266003, China |
| Abstract: | OBJECTIVE To observe the protective effect of Haikangling (HKL-serum)on neuroblastoma SH-SY5Y cells.METHODS The model of H_2O_2-induced neuroblastoma SH-SY5Y cells in vitro was established, and the protective effect of HKL-serum was investigated by observing the morphology of cells, detecting the survival rate and the LDH release. RESULTS Compared with the model group,HKL-serum(10% and 15%) attenuated the impairment in H_2O_2-induced neuroblastame SH-SY5Y cells by increasing significantly the survival rate(P<0.01),lowering the LDH release from cells into culture medium(P<0.01)and ameliorating the cell morphology. CONCLUSION HKL-serum has significantly protective effects on H_2O_2-induced impairment in neuroblastoma. |
| Keywords: | H2O2 |
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