| HPLC法测定跌打巴布剂中三七皂苷R1和人参皂苷Rg1含量 |
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| 引用本文： | 陈文珠,袁小红.HPLC法测定跌打巴布剂中三七皂苷R1和人参皂苷Rg1含量[J].中国医药指南,2013,0(9):62-63. |
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| 作者姓名： | 陈文珠 袁小红 |
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| 作者单位： | 陈文珠 (广东省中医院,广东 广州 510120)； 袁小红 (广东省中医院,广东 广州 510120)； |
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| 摘 要： | 目的建立跌打巴布剂中三七皂苷R1和人参皂苷Rg1的含量测定方法。方法采用HPLC法,以Agilent TC-C18ODS柱为色谱柱,以乙腈-水(24:76)为流动相,检测波长203nm,流速1mL/min。结果三七皂苷R1线性范围为0.583~29.15μg(r=0.9999,n=5),回收率为99.9%~100.3%。人参皂苷Rg1线性范围为0.336~16.8μg(r=0.9999,n=5),回收率为99.8%~100.2%。结论本方法准确、可靠、灵敏度高,可用于跌打巴布剂的定量检测。
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| 关 键 词： | 跌打巴布剂 高效液相色谱法 三七皂苷R1 人参皂苷Rg1 |
HPLC Determination of Notoginsenoside R1 and Ginsenoside Rg1 in Dieda Cataplasm |
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| CHEN Wen-zhu,YUAN Xiao-hong.HPLC Determination of Notoginsenoside R1 and Ginsenoside Rg1 in Dieda Cataplasm[J].Guide of China Medicine,2013,0(9):62-63. |
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| Authors: | CHEN Wen-zhu YUAN Xiao-hong |
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| Institution: | (Guangdong Provincial Hospital of Traditional Chinese Medicine,Guangzhou 510120,China) |
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| Abstract: | Objective Determine notoginsenoside R1 and ginsenoside Rg1 in Dieda cataplasm by HPLC.Methods Agilent TC-C18 ODS column was used with the mobile phase containing acetonitrile-water（24：76）.The wavelength of detector was set at 203nm.The flow rate was 1mL/min.Results The calibration curves were linear in the range of 0.583~29.15μg for notoginsenoside R1（r=0.9999,n=5） and 0.336~16.8μg for ginsenoside Rg1（r=0.9999,n=5）.The recovery of notoginsenoside R1 was 99.9%~100.3%,and that of ginsenoside Rg1 was 99.8%~100.2%.Conclusion The method is accurate,reliable and simple,and is suitable for the determination of Dieda cataplasm. |
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| Keywords: | Dieda cataplasm HPLC Notoginsenoside R1 Ginsenoside Rg1 |
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