对苯二甲酸对NIH-3T3细胞毒性的研究

目的：探讨对苯二甲酸的细胞毒性，方法：应用MTT比色法及电镜技术，以NIH－3TE为靶细胞。结果：对苯二甲酸浓度在156．25－625μg/ml范围内，短时间内对细胞生长有刺激作用，随着染毒时间的延长及剂量的加大，细胞生长出现抑制，并有一定的剂量依赖趋势。电镜检测结果显示，各染毒组细胞形态增大，胞内出现大量空泡，自噬体及髓样变；在浓度达到625μg/ml时，核分裂相明显增多。结论：对苯二甲酸具有一定的细胞毒性，其在TPA致膀胱肿瘤的机制研究中具有重要意义。

Study on the toxicity of terephthalic acid to NIH-3T3 cells

Objective To study the cytotoxicity of Terephthalic acid(TPA).Methods MTT assay and electron micrograph were performed,the NIH-3T3 cell line was selected as target cell.Results TPA ranged from 156.25 to 625 μg/ml could irritate cell growth in short time;the cell growth was inhibited with the increase of exposure dose and exposure time.The electron micrograph showed that the cell morphology was changed in all exposing groups,including cell enlargement,vacuolization,autophagic body and myeloid change,and karykinesis phase was also increased when TPA dose reached 625μg/ml.Conclusion TPA shows some cytotoxicity which has some importance in the pathogenesis of bladder tumor.