| 乙酰胆碱诱导的离体兔心房颤动模型的建立 |
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| 引用本文： | 潘磊,李俊.乙酰胆碱诱导的离体兔心房颤动模型的建立[J].同济大学学报(医学版),2012,33(3):33-36. |
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| 作者姓名： | 潘磊 李俊 |
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| 作者单位： | 同济大学东方转化医学研究中心,上海200120;教育部同济大学心律失常重点实验室,上海200120 |
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| 基金项目： | 国家自然科学基金，上海市自然科学基金项目 |
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| 摘 要： | 目的建立乙酰胆碱诱导的离体Langendorff灌流的兔的心房颤动模型。方法健康雄性的新西兰兔40只,2.2～2.5kg分为4组,分别为正常灌流组、正常灌流+burst刺激组、正常灌流+乙酰胆碱灌注组和正常灌流+乙酰胆碱灌注+burst刺激组,每组各10只。在Langendorff灌流装置下行主动脉逆行灌流,通过乙酰胆碱灌注和burst刺激诱发心房颤动。结果正常灌流组、正常灌流+burst刺激组和正常灌流+乙酰胆碱灌注组均没有发生心房颤动,而正常灌流+乙酰胆碱灌注+burst刺激组心房颤动的发生率为50%。结论在Langendorff心脏灌流时,给予乙酰胆碱灌注模拟胆碱能激活,并给予burst刺激模拟应激事件,可以稳定地诱导心房颤动的发生。
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| 关 键 词： | 心房颤动 乙酰胆碱 新西兰兔 |
Establishment of acetylcholine-induced atrial fibrillation model in isolated rabbit heart |
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| PAN Lei and LI Jun.Establishment of acetylcholine-induced atrial fibrillation model in isolated rabbit heart[J].Journal of Tongji University(Medical Science),2012,33(3):33-36. |
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| Authors: | PAN Lei and LI Jun |
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| Institution: | 1,2) (1.Oriental Medical Translational Research Center of Tongji University,Shanghai 200120,China; 2.Key Laboratory of Arrhythmias Studies,Ministry of Education at Tongji University,Shanghai 200120,China) |
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| Abstract: | Objective To establish an acetylcholine-induced atrial fibrillation(AF)model in the isolated rabbit heart.Methods Forty healthy male New Zealand rabbits(2.2～2.5kg)were used in the study.The isolated rabbit hearts were randomly assigned to normal perfusion(group A),normal perfusion with burst high-frequency electric stimulation(group B),acetylcholine perfusion(group C) and acetylcholine perfusion with burst electric stimulation(group D)(n=10 in each group).The isolated hearts were perfused with Langendorff perfusion system.Results The AF incidence in group D(acetylcholine perfusion combined with burst electric stimulation)was 50%;while no AF was induced in other three groups.Conclusion A simple and stable acetylcholine-induced atrial fibrillation model has been established in isolated rabbit heart with Langendorff perfusion system. |
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| Keywords: | atrial fibrillation acetylcholine New Zealand rabbit |
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