Bcl-2高表达小鼠模型的建立

目的 建立Bcl-2高表达的转基因小鼠品系,为在活体动物上研究bcl-2蛋白的功能提供动物模型.方法 构建含人Bcl-2基因的质粒;通过显微注射将该质粒转入C57BL/6J×CBA的杂交小鼠的受精卵,并植入假孕母鼠的子宫,PCR检测后携带人Bcl-2基因的小鼠即为“Founder”鼠;将“Founder”鼠与C57BL/6J×CBA杂交小鼠不断回交,经PCR和Western bilotting筛选后即可获得阳性小鼠.结果 共获得5只“Founder”鼠,其中#6、#14成功建系,连续回交繁殖7代后,PCR和Western筛选后获得bcl-2高表达阳性小鼠42只.结论 成功将人bcl-2基因转入小鼠基因组内,并稳定传代,建立Bcl-2高表达小鼠,为进一步研究Bcl-2蛋白提供了很好的动物模型.

Establishment of mouse model highly expressing Bcl-2 protein

Objective To establish a mouse model highly expressing Bcl-2 protein for study of Bcl-2 function in vivo.Methods The plasmid containing human bcl-2 gene was constructed,which was transferred to fertilized eggs of C57BL/6J×CBA hybrid mice by microinjection;the fertilized eggs were implanted in the uterus of falsely pregnant female mice.The bcl-2 gene-carrying mice were the "Founder" mice,the "Founder" mice and C57BL/6J×CBA hybrid mice were continuously backcrossed,and the transgenic mice expressing Bcl-2 were obtained by screening with PCR and Western blotting.Results Five "Founder" mice were obtained;the strains were successfully established from #6 and #14 mice.After 7 generations of backcross breeding and screening by PCR and Western blot,42 transgenic mice highly expressing bcl-2PCR were obtained.Conclusion The mouse model highly expressing Bcl-2 protein has been successfully established in this study.