三种金属烤瓷冠修复后龈沟液中MMP-2水平检测

目的 研究镍铬合金、钴铬合金、金合金三组金属烤瓷冠修复前后,龈沟液(gingival crevicular fluid,GCF)中基质金属蛋白酶-2(matrix metalloproteinase,MMP-2)水平变化,提示三种合金烤瓷冠在不同时期对牙周组织的刺激程度.方法 临床随机选择三种金属烤瓷冠修复患者共45例,每组各15例,测定在修复前、修复后1个月、3个月、6个月GCF量并采用双抗体夹心酶联免疫吸附法(ABC-ELISA)检测同期GCF中MMP-2水平.结果 镍铬合金烤瓷冠GCF量、MMP-2水平在不同检测周期差异有显著性(P＜0.01),钴铬合金、金合金烤瓷冠修复后MMP-2水平在不同检测周期差异无显著性(P＞0.05).结论 MMP-2可作为对不同金属烤瓷冠内冠材料刺激牙周组织的评价指标,临床应采用生物相容性好的合金作为内冠材料.

Concentrations of MMP-2 in gingival crevicular fluid of porcelain teeth with three different alloys

Objective To measure MMP-2 concentrations in gingival crevicular fluid(GCF)of porcelain teeth coated with Ni-Cr,Co-Cr or gold alloy at different time period,and to demonstrate the degree of stimulation by these alloy on gingivae.Methods Sandwich enzyme-linked immunosorbent assay(ELISA)was used to determine the concentration and total amount of MMP-2 in gingival crevicular fluids in 45 randomly selected cases of porcelain teeth coated with Ni-Cr,Co-Cr or gold alloy,with 15 cases in each group.The assay was performed before and at 1,3,6 months after treatment.Results There was significant difference in the total amounts of MMP-2 and GCF volume for the Ni-Cr alloy coated porcelain teeth in different time period(P<0.01).However,there was no significant difference in MMP-2 concentrations between Co-Cr and gold alloy coated porcelain teeth at different time periods(P>0.05).Conclusion MMP-2 is a useful assessment criterion for the evaluation of degree of stimulation on gingivae by different alloy coating materials in porcelain teeth. The bio-compatibility of alloy coating for porcelain teeth should be carefully considered for the clinical application.