| 姜黄素单体影响肝癌BEL-7402细胞凋亡及其周期蛋白D表达的研究 |
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| 引用本文： | 宛传丹,温占朝,顾勇劲.姜黄素单体影响肝癌BEL-7402细胞凋亡及其周期蛋白D表达的研究[J].重庆医学,2011,40(23). |
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| 作者姓名： | 宛传丹 温占朝 顾勇劲 |
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| 作者单位： | 宛传丹,Wan Chuandan(江苏省常熟市医学检验所,215500);温占朝,顾勇劲,Wen Zhanchao,Gu Yongjin(江苏省常熟市第二人民医院,215500) |
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| 摘 要： | 目的 采用细胞学实验观察3种姜黄素单体对肝癌细胞株BEL-7402的抑制增殖及诱导细胞凋亡的作用强度和机制.方法 以四甲基偶氮唑蓝(MTT)、Annexin Ⅴ-FITC双标记及流式细胞术(FCM)实验观察3种姜黄素对肝癌细胞株BEL-7402增殖的抑制作用与细胞周期变化,采用 Western blot实验分析3种姜黄素对肝癌细胞株BEL-7402细胞周期蛋白D表达的影响.结果 (1)3种姜黄素均可通过诱导肝癌细胞凋亡而有效抑制肝癌细胞生长增殖,存在时间与剂量效应,以姜黄素Ⅲ抑制效果最强.(2)3种姜黄素通过影响细胞周期生长的调控信号、降低细胞周期蛋白D表达量致使肝癌细胞停滞于G1/S期.结论 3种姜黄素均可通过抑制肝癌细胞周期蛋白D表达而诱导细胞凋亡,有效地抑制肝癌细胞生长增殖,具有很高的临床治疗价值.
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| 关 键 词： | 姜黄素 肝肿瘤 细胞凋亡 细胞周期蛋白D |
Effects of curcumins on cyclin D expression and apoptosis of human hepatoma BEL-7402 cells |
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| Wan Chuandan,Wen Zhanchao,Gu Yongjin.Effects of curcumins on cyclin D expression and apoptosis of human hepatoma BEL-7402 cells[J].Chongqing Medical Journal,2011,40(23). |
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| Authors: | Wan Chuandan Wen Zhanchao Gu Yongjin |
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| Abstract: | Objective To study the effects of three kinds of curcumins on proliferation inhibition and apoptosis induction of the cell line of human hepatoma BEL-7402 and to elucidate their possible molecular mechanism.Methods The inhibiting ratio of 3 curcumins in different concentrations were determined by MTT assay at different times.Apoptosis was evaluated by agarose gel electrophoresis and Annexin V-FITC double pigmentation method.The cell cycle was detected by FCM.Western blot method were used to analysis the exp... |
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| Keywords: | curcumin liver neoplasms apoptosis cyclin D |
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