| 高效液相色谱法测定五加参颗粒中紫丁香苷含量 |
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| 引用本文： | 彭敏,谭洁.高效液相色谱法测定五加参颗粒中紫丁香苷含量[J].中国药业,2012,21(4):39-40. |
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| 作者姓名： | 彭敏 谭洁 |
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| 作者单位： | 1. 湖南省湘西土家族苗族自治州药品检验所,湖南吉首,416000
2. 南华大学药学与生命科学学院,湖南衡阳,421000 |
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| 摘 要： | 目的 建立测定五加参颗粒中紫丁香苷含量的高效液相色谱法.方法 采用Agilent C18色谱柱(250mm×4.6mm,5 μm),流动相为乙腈-0.3%甲酸溶液(体积比12:88),流速为1.0 mL/min,检测波长为265 nm,柱温为30℃.结果 紫丁香苷进样量在0.041 04～0.451 4μg(r=0.999 9)范围内与峰面积积分值线性关系良好,平均加样回收率为101.50%,RSD=1.96%(n=6).结论高效液相色谱法灵敏度高、操作简便、准确可靠,可用于五加参颗粒中紫丁香苷的含量测定.
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| 关 键 词： | 紫丁香苷 高效液相色谱法 含量测定 |
Determination of Syringin Content in Wujiasheng Granules by HPLC |
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| Peng Min,Tan Jie.Determination of Syringin Content in Wujiasheng Granules by HPLC[J].China Pharmaceuticals,2012,21(4):39-40. |
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| Authors: | Peng Min Tan Jie |
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| Institution: | 1. Institute for Drug Control of Xiangxi Tujia - Miao Autonomous Prefecture, Jishou, Hunan, China 416000; 2. College of Pharmaceutical and Life Sciences, Nanhua University, Hengyang, Hunan, China 421000 ) |
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| Abstract: | Abstract：Objective To develop a HPLC method for the determination of syringin in Wujiasheng Granules. Methods The Agilent C18 column（250 mm×4.6 mm,5 μm）was used with the mobile phase of acetonitrile-0.3% formic acid （12：88） at the flow rate of 1.0 mL/min. The detection wavelength was 265 nm and the temperature was 30 ℃. Results The good linear relation of syringin was in the injection volume of 0. 041 04-0. 451 4 μg（r = 0. 999 9）. The average recovery rate was 101.50%, RSD = 1.96% （n = 6）. Conclusion The method is sensitive,convenient and accurate,which can be used to determine the content of syringin in Wujiasheng Granules. |
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| Keywords: | syringin HPLC content determination |
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