红树林植物内生放线菌I07A-01824发酵液中杀线虫活性成分的分离、纯化与鉴定

目的建立稳定有效的体外杀线虫模型,并从红树林植物木榄内生放线菌I07A-01824的发酵液中分离纯化具有杀线虫活性的物质,并鉴定其化学结构。方法在体外杀线虫模型指导下,利用硅胶层析、高效液相色谱等方法对放线菌I07A-01824的发酵液进行分离纯化,得到高纯度的杀线虫活性物质;通过现代波谱分析技术对其进行结构解析,确定其化学结构。结果红树林内生放线菌I07A-01824的次级代谢产物中具有较强杀线虫活性的物质,为一不饱和脂肪酸,化学结构为5,8-二烯十四酸;27.3mg/L剂量下开始显示对秀丽隐杆线虫有活性,其24h后的LC50值为162.8mg/L。结论此不饱和脂肪酸对线虫有较强的致死作用,并且在内生放线菌I07A-01824菌株的次级代谢产物中稳定存在。

Separation, purification and structural identification of an active nematicidal component from the fermentation broth of Endophytic streptomyces I07A-01824 from Mangrove ecosystem

Objective To establish an efficient in vitro model for screening of new nematicidal compounds,and to separate,purify and identify the structure of an active nematicidal component from the fermentation broth of Endophytic actinomycets I07A-01824.Methods Silica gel column chromatography and HPLC were used to separate and purify the active component.Its structure was identified by spectroscopic analyses.Results The active component was purified and its structure was identified as an unsaturated fatty acid-tetradeca-5,8-dienoic acid.It displays the cytotoxic activity to Caenorhabditis elegans at 27.3 mg/L.Its LC50 is 162.8 mg/L in 24 h.Conclusions The unsaturated fatty acid was first isolated from the strain of Endophytic actinomycets with nematicidal activity.