大肠癌患者VEGF和NO血清水平及其与恶变程度的关系

目的:研究血清中血管内皮生长因子(VEGF)和一氧化氮(NO)表达水平及其与大肠癌恶变程度的关系。方法:分别采用酶联免疫吸附法(ELISA)测定和分光光度法检测46例大肠癌患者术前和30例健康人血清中VEGF和NO的含量。结果:大肠癌患者血清VEGF和NO表达水平均较健康人明显增高(P<0.01),且随着大肠癌浸润深度增加、有淋巴结转移以及Dukes分期愈晚者而显著增高(P<0.01)。血清VEGF与NO含量呈明显正相关(r=0.8152,P<0.01)。结论:VEGF和NO与大肠癌的发生、发展及转移调控过程有关,术前检测血清VEGF和NO含量对判断大肠癌的浸润转移以及Dukes分期具有重要价值。

Relationship between Serum Level of VEGF/NO and Extent of Malignancy in Patients with Colorectal Carcinoma

Objective: To study the serum vasular endothelial growth factor (VEGF) and nitric oxide (NO) levels in patients with colorectal cancer and their relationship with the malignancy.Method: Serum VEGF level was measured by enzyme linked immunosorbent assay (ELISA) and serum NO level was detected by spectrophotometry in 46 colorectal cancer patients and 30 healthy individuals.Results: Serum levels of VEGF and NO in colorectal cancers patients were significantly higher than those of in normal persons (P<0.01). There was a positive correlation between serum VEGF, NO levels and tumor invasion depth, lymph node metastasis and Dukes stage (P<0.01). There was also a positive correlation between serum VEGF level and serum NO level (r=0.8152,P<0.01).Conclusion: VEGF and NO are related to the growth, development and metastasis of colorectal cancer. Serum levels of VEGF and NO in colorectal cancer patients may be a sensitive predictor of invasion, metastasis and Dukes stage.