| SiO2刺激矽肺肺泡巨噬细胞对MMP-9和TIMP-1表达的影响 |
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| 引用本文： | 张丽,郝小惠,王献华,杨方,郭志义,裴鑫. SiO2刺激矽肺肺泡巨噬细胞对MMP-9和TIMP-1表达的影响[J]. 中华劳动卫生职业病杂志, 2005, 27(1): 341-344. DOI: 10.3760/cma.j.issn.1001-9391.2009.06.007 |
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| 作者姓名： | 张丽 郝小惠 王献华 杨方 郭志义 裴鑫 |
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| 作者单位： | 天津市人民医院病理科,300121;华北煤炭医学院实验中心; |
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| 摘 要： | Objective To investigate the effect of SiO2 on the expression of matrix metalloproteinase-9 (MMP-9)and tissue inhibitor of metalloproteinase-1 (TIMP-1)in human silicofic alveolar macrophages (AM).Methods Human alveolar macrophages were collected from a silicotic patient by bronchoalveolar lavage and exposed to silicon dioxide for 3h,6h,12h,18h,24h and 36h.The expression of the MMP-9 in the AM were detected with zymography and immunological method and the expression of the TIMP-1 in the AM with immunological method.Results The expressions of MMP-9 in the AM increased clearly along with the time,reached peak at 24 h when detected with zymography (average optical density:3.061±0.153 vs 2.851±0.164,P＜0.05);and reached peak at 18h when detected with immunological method (average optical density:0.386±0.037 vs 0.322±0.034,P＜0.05).The expression of the TIMP-1 in the AM did not vary when detected with immunological method (P＞0.05).Conclusion SiO2 may affect the expression of MMP-9 and MMP-9 activity in the cultured AM.
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| 关 键 词： | 二氧化硅 矽肺 巨噬细胞,肺泡 基质金属蛋白酶 |
Effect of SiO2 on expression of MMP-9/TIMP-1 in human alveolar macrophages in vitro |
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| ZHANG Li,HAO Xiao-hui,WANG Xian-hua,YANG Fang,GUO Zhi-yi,PEI Xin. Effect of SiO2 on expression of MMP-9/TIMP-1 in human alveolar macrophages in vitro[J]. Chinese journal of industrial hygiene and occupational diseases, 2005, 27(1): 341-344. DOI: 10.3760/cma.j.issn.1001-9391.2009.06.007 |
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| Authors: | ZHANG Li HAO Xiao-hui WANG Xian-hua YANG Fang GUO Zhi-yi PEI Xin |
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| Abstract: | |
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| Keywords: | Silicon dioxideSilicosisMacrophages alveolarMatrix metalloproteinases |
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