rhIFN-a对增生性瘢痕成纤维细胞超微结构影响的实验研究

目的研究细胞因子rhIFN-a对增生性瘢痕成纤维细胞超微结构的影响,以探讨细胞因子对增生性瘢痕的作用机理.方法对增生性瘢痕成纤维细胞进行体外培养,采用透射电子显微镜观察、分析,以研究细胞因子rhIFN-a对增生性瘢痕成纤维细胞生物学行为的影响以及病理改变.结果加入rhIFN-a后细胞表现为生长趋势及增殖活性被明显抑制,部分细胞见空泡样及残体样结构,线粒体肿胀,嵴溶解,并出现细胞凋亡现象.结论细胞因子rhIFN-a对成纤维细胞的生长趋势及增殖活性有明显抑制作用,促进成纤维细胞凋亡.提示rhIFN-a是成纤维细胞负性调节因子,在增生性瘢痕的治疗中有一定的应用价值.

Effect of rhIFN - a on Ultrastructure of Hypertrophic Scar Fibroblasts

Objective To investigate effects cytokine of rhIFN-a and their on ultrastructure of hypertrophic scar derived fibroblasts and explore its mechanism.Methods Hypertrophic scar fibroblasts cultivated in vitro were observed and analysed through transmission elextron microscope(TEM).Then the effects of rhIFN-a on biological behavior and pathological changes of hypertrophic scar fibroblasts have been determined.(Results) Compared with the control group,the experimental rhIFN-a group could be observed changes in inhibition of fibroblasts growth and proliferation with the mitochondrion swelling,its ridge dissolving and apoptosis promoting.Conclusions rhIFN-a can suppress growth and proliferation of hypertrophic scar fibroblasts and promotes apoptosis.This indicates that ,as a negative modulation factor,rhIFN-a is important in the treatment of hypertrophic scar.