PCR技术在儿童ALL微小残留病中的检测及临床意义

目的：探讨ＰＣＲ检测ＡＬＬ－ＭＲＤ的重要性及临床意义。方法：运用ＰＣＲ技术，以ＩｇＨ和ＴｅＲγ基因重排作为标志，检测了４０例急性淋巴细胞白血病（ＡＡＬ）微小残留病（ＭＲＤ）。结果：７例初治未缓，１２例部分缓解（ＰＲ），１３例完全缓解（ＣＲ）的ＡＬＬ患儿ＭＲＤ检测阳性为另８例（１例ＰＲ、７例ＣＲ）ＭＲＤ阴性。发现ＣＲ时间赵长，ＭＲＤ阳性率越低，反之，ＣＲ时间越短，ＭＲＤ阳性率越高，结论：ＭＲＤ何作为

Detection of Minimal Residual Disease in Childhood Acute Lymphocytic Leukemia by Polymerase Chain Reaction and Clinical Significance

To detect minimal residual disease(MRD) of 40 cases of childhood acute lymphocytic leukemia(ALL) by polymerase chain reaction,the IgH and TCRY generearrangements were regardes as marker. Results in 7 cases unremission (NR ), 12 cases partrial remission (PR) and 13 cases complete remission (CR) ALL patients,their MRD was positive, another 8 cases (1 case PR. 7 cases CR) MRD was negative,which showed that the complete remission time was more long,the MRD positive rate was more lower,the time was more short,the positive rate was more higher. The study suggested that MRD was closely associated with leukemia relapse,the detection of MRD could guide effectively clinical continuative treatment.