可注射性组织工程骨的研究

目的已经有实验证明可用组织工程方法构建可注射性软骨,试图应用组织工程技术探索可注射性组织工程骨的可行性。方法从兔髂骨骨髓细胞中获取骨髓基质成骨细胞,将骨须基质成骨细胞与2.0%藻酸钠溶液混合形成骨髓基质成骨细胞/藻酸钠复合物,使细胞的终浓度为5×106/ml。将骨髓基质成骨细胞/藻酸钠复合物注射于裸鼠背部皮下,用氯化钙固化为凝胶体。结果注射后4,8周注射物在裸鼠背部皮下形成硬结节,X线片均显示有明显成骨现象。组织学分析显示标本有大量新骨形成,并具有髓腔样结构。结论应用藻酸钠复合骨髓基质成骨细胞可通过注射方式在动物体内形成骨组织。

A study of injectable tissue-engineered bone

Aim It has been proved that we can construct injectable tissue engineered cartilage.The study investigates the utilization of alginate carrier for delivering osteoblasts and creating new bone tissue in nude mice model via injection.Methods Bone marrow cells harvested from iliac bone of New Zealand rabbits differentiate into bone stromal osteoblasts.The osteoblasts were mixed with 2.0％ alginate sodium solution to generate osteoblasts/alginate composites with final cellular density of 5× 106/ml.Calcium chloride was used as cross-linking agent to gel aqueous alginate solution.The osteoblasts/alginate composites were injected into the dorsal subcutaneous tissue of nude mice.Results Four and eight weeks after injection, the hard knobbles were easily palpated under the dorsal skin of animals.X-ray photograph showed that the knobbles has calcified image with more density than sounding soft tissue.In histological analysis, new bone formation was observed in the osteoblasts/alginate composites.The osteogenesis was in association with regenerated hematopoietic bone marrow.Conclusion These results revealed that new bone tissue could be created through the injection of alginate sodium mixed with marrow stromal osteoblasts.