HIV早期检测的应用研究 |
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引用本文: | 刘萃红,朱琳,宋一帆.HIV早期检测的应用研究[J].中国现代医生,2008,46(34):7-8. |
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作者姓名: | 刘萃红 朱琳 宋一帆 |
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作者单位: | 1. 郑州大学第一附属医院检验科,郑州,450052 2. 郑州大学第一附属医院药剂科,郑州,450052 3. 北京大学医学部,北京,100083 |
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基金项目: | 河南省医学科技创新人才工程项目 |
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摘 要: | 目的采用抗HIV-1 p24单克隆抗体和多克隆抗体构建的间接ELISA试剂,检测HIV-1抗体阳性及其他样品,以探讨HIV早期检测的可行性。方法采用单克隆抗体固相、兔抗HIV-1 p24抗体夹心,羊抗兔HRP结合物的间接ELISA法。结果显示试剂盒HIV-1 p24抗原检出灵敏度为50pg/mL(基因工程抗原);特异性97.13%;HIV抗体阳性血样阳性率4.1%;抗体阴性的特殊人群血样阳性率5.2%;抗体不确定血样阳性率为16.3%,明显高于抗体阳性和阴性组血样。病毒培养1d,抗原效价1:80,第3天可达1:5360。结论该间接夹心HIV-1 p24抗原检测试剂,可应用于HIV感染的辅助检测及病毒的基础研究,具有灵敏度高、特异性好的特点。
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关 键 词: | HIV ELISA 单克隆抗体 |
A Study on Applications of the Early Detection of HIV |
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Authors: | LIU Cuihong ZHU Lin SONG Yifan |
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Institution: | LIU Cuihong1 ZHU Lin2 SONG Yifan3 1.Department of Laboratory,The First Affiliated Hospital of Zhengzhou University,Henan 450052,2.Department of Pharmacy,3. Peking University Health Science Center,Beijing 100034 |
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Abstract: | Objective To determine the samples which are HIV-1 Antibody positive and others by using the indirect Sandwich ELISA kit constructed by HIV-1 p24 monoclonal antibody and polyclonal antibody. Methods We used double antibody enzyme linked immunosorbent assay(ELISA) to test different samples. Results Sensitivity and specificity of the kit were 50pg/mL(recombinant antigen) and 97.13% respectively. Among the different samples 4.1% of anti-HIV positive,5.2% of high risk group populations and 16.3% of anti-HIV ind... |
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Keywords: | HIV EHSA |
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