TGFβ对胃癌细胞浸润、转移的影响及其作用机制研究

目的:探讨TGFβ与胃癌浸润、转移之间的关系及其作用机制。方法:以胃癌细胞株SGC7901和BGC823为对象，应用transwell迁移浸润、matrigel fibronectin基质黏附、内皮细胞黏附、明胶酶谱及裸鼠成瘤等实验分别检测0 ng/mL或10 ng/mL TGFβ1处理前后胃癌细胞株生物学行为的变化。结果:经TGFβ1处理成瘤裸鼠4周后肝转移数量远高于未处理组（P<0.05）; SGC7901和BGC823细胞经TGFβ1处理后，瘤细胞浸润基质胶的能力增强; 瘤细胞的迁移、黏附能力亦明显增加。结论:TGFβ1促进胃癌浸润、转移，其机制可能与其促进瘤细胞运动、黏附以及基质金属蛋白酶分泌有关。

The effect of transforming growth factor-β1 on the metastatic and in vitro invasive capacity of gastric cancer cells and its mechanism

Abstract:Objective:To investigate the effect of transforming growth factor-β1 on metastasis and invasion of gastric cancer and its mechansism.Methods :The changes of bilogical behaver of gastric cancer SGC7901 and BGC823 cell line were tested after pretreatment by TGFβ1 at 0 or 10 ng/mL,and liver metastasis was measured 4 weeks after inoculation in Balb/c nude mice. Results:SGC7901 and BGC823 cells treated with TGF-β1 had more hepatic metastases and the effect of penetrated reconstituted basement-membrane barriers increased significantly than that untreated control cells did. Likewise, there was an apparent increase in tumor cell migration and adhesion capacity after treating with TGFβ1.Conclusions:These results suggest that TGF-β1 may modulate metastatic potential of gastric cancer cells by promoting their ability to break down and penetrate basement membrane barriers and by their adhesive and motile activities. TGF-β1 acts on SGC7901 and BGC823 cells as a progression-enhancing factor.