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| p16和p53基因及顺铂联用对胆管癌细胞系QBC939的生长抑制作用 | |
| 引用本文： | 鲁建国,林晨,黄志强,马庆久,褚延魁,何显力,付明,张雪艳,梁萧,要秀,吴旻.p16和p53基因及顺铂联用对胆管癌细胞系QBC939的生长抑制作用[J].第四军医大学学报,2002,23(21):1923-1925. |
| 作者姓名： | 鲁建国 林晨 黄志强 马庆久 褚延魁 何显力 付明 张雪艳 梁萧 要秀 吴旻 |
| 作者单位： | 1. 第四军医大学唐都医院普外科,陕西,西安,710038 2. 中国医学科学院,中国协和医科大学肿瘤研究所分子肿瘤学国家重点实验室,北京,100021 3. 解放军总医院普外研究所,北京,100853 |
| 基金项目： | 863高科学技术发展资助项目 (Z2 0 -0 1-0 2 ) |
| 摘 要： | 目的：探索p16基因和p53基因及p16基因和顺铂（CDDP）联合应用对体内外胆管癌细胞系生长的抑制作用。方法：将重组体腺病毒p16（Ad-p16）和Ad-p53及Ad-p16和CDDP联合作用于人胆管癌细胞系QBC939，观察和分析其对体内外胆管癌细胞生长抑制作用及其机制，结果：Ad-p16在QBC939细胞中表达能抑制QBC939细胞的生长，与p53、CDDP联合应用能明显抑制QBC939细胞的生长，p16和p53基因诱导癌细胞发生G1期阻滞和细胞凋亡，CDDP能诱导癌细胞发生G2期阻滞和细胞凋亡；裸鼠皮下移植瘤模型瘤体内注射Ad-p16及腹腔内注射CDDP均能抑制QBC939肿瘤的生长，两者联合应用具有协同作用。结论：p16基因和p53基因及CDDP联合应用具有协同作用。 |
| 关 键 词： | 胆管癌 p16基因 p53基因 腺病毒 顺铂 基因治疗 动物模型 CDDP |
| 文章编号： | 1000-2790(2002)21-1923-03 |
| 修稿时间： | 2002年8月28日 |
Inhibitory effects of gene p16 together with gene p53 and cisplatin to the growth of cholangiocarcinoma cell line QBC939 |
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| LU Jian Guo ,LIN Cheng ,HUANG Zhi Qiang ,MA Qin Jiu ,CHU Yan Kui ,HE Xian Li ,FU Ming ,ZHANG Xue Yan ,LIANG Xiao ,YAO Xiu ,WU Wen.Inhibitory effects of gene p16 together with gene p53 and cisplatin to the growth of cholangiocarcinoma cell line QBC939[J].Journal of the Fourth Military Medical University,2002,23(21):1923-1925. | |
| Authors: | LU Jian Guo LIN Cheng HUANG Zhi Qiang MA Qin Jiu CHU Yan Kui HE Xian Li FU Ming ZHANG Xue Yan LIANG Xiao YAO Xiu WU Wen |
| Institution: | LU Jian Guo 1,LIN Cheng 2,HUANG Zhi Qiang 3,MA Qin Jiu 1,CHU Yan Kui 1,HE Xian Li 1,FU Ming 2,ZHANG Xue Yan 2,LIANG Xiao 2,YAO Xiu 1,WU Wen 2 1Department of General Surgery,Tangdu Hospital,Fourth Military Medical Univer |
| Abstract: | AIM To explore inhibitory effects of recombinant adenoviruses p16 (Ad p16) with Ad p53 and Ad p16 with cisplatin (CDDP) to the growth of cholangiocarcinoma cell. METHODS The effects of Ad p16 with Ad p53 and Ad p16 with CDDP on the growth QBC939 cells in vitro and in vivo were analyzed. RESULTS Ad p16 expressed in cholangiocarcinoma cell line can inhibit growth of the QBC939 cells, while Ad p16 with Ad p53 and Ad p16 with CCDP together applied could significantly inhibit the growth of QBC939 cell in vitro and in vivo . The p16 with p53 transfected cells manifested apoptosis and G1 arrest, while CCDP induced G2 arrest and apoptosis. CONCLUSION p16 with p53 and p16 with CDDP can obviously increased the suppresson effeets on human cholangiocarcinoma cell line. |
| Keywords: | cholangiocarcinoma p16 gene p53 gene adenovirus cisplatin gene therapy |
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