Effect of mammalian thyrotropin releasing hormone on prolactin secretion by bullfrog adenohypophyses in vitro. |
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Authors: | G K Clemons S M Russell C S Nicoll |
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Affiliation: | Department of Physiology-Anatomy, University of California, Berkeley, California 94720 USA |
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Abstract: | The effects of the mammalian thyrotropin-releasing hormone (TRH) on the secretion of prolactin by bullfrog adenohypophysis in vitro were investigated in short-term incubation and 24-hr organ culture experiments. Prolactin in the medium or in the incubated tissue was measured by either polyacrylamide disc gel electrophoresis and densitometry or by a homologous radioimmunoassay. The TRH was consistently effective in promoting prolactin release in vitro in concentrations of 10 ng/ml to 10 μg/ml. The tripeptide also caused an increase in the tissue prolactin content over a wide range of concentrations. These results indicate that TRH may function as a prolactin-releasing factor in the bullfrog. |
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