| 应用微菌落技术对细菌进行快速定量测定 |
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| 引用本文： | 许欣 康莉. 应用微菌落技术对细菌进行快速定量测定[J]. 中国卫生检验杂志, 1995, 5(1): 9-11 |
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| 作者姓名： | 许欣 康莉 |
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| 作者单位： | 华西医科大学公共卫生学院，华西医科大学公共卫生学院，华西医科大学公共卫生学院，华西医科大学公共卫生学院卫检专业，成都军区总医院检验科，华西医科大学公共卫生学院 四川成都 610041，四川成都 610041，四川成都 610041，87级学生，四川成都 610041 |
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| 摘 要： | 本文利用微菌落技术建立了一种对细菌进行快速定量测定的方法，其基本方法为将待测标本定量接种于醋酸纤维素薄膜上，经３７℃５ｈ培养，染色后对标本区的微菌落作显微镜计数，用本法与平板计数法同时对大杆菌和金黄色葡萄球菌悬液进行测定，得到很好的相关性，对７２份尿液标本测定结果作统计学分析，二者差别无显著性。
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| 关 键 词： | 微菌落 细菌 定量测定 卫生检验 |
Rapid Enumeration of Bacteria by Microcolony Technique |
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| Xu Xin Pei Xiaofang Ye MeijunCollege of Public Health,West China University of Medical Sciences. Rapid Enumeration of Bacteria by Microcolony Technique[J]. Chinses Journal of Health Laboratory Technology, 1995, 5(1): 9-11 |
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| Authors: | Xu Xin Pei Xiaofang Ye MeijunCollege of Public Health West China University of Medical Sciences |
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| Affiliation: | Chengdu 610041 |
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| Abstract: | This paper described a microcolony method for rapid enumeration of bacteria. In brief, a certainamount of samples were inoculated on the cellulose ?**,acetate membrane which had been placed on the incubation pad- Aftt-r incubated for 5h at 37 C. the membrane was dried and stained. The number of micro-colonies were counted under the microscope- The microcolony count of bacteria on the membrane correlated well with the plate count of E. coli, S. aureus and bacteria in urine. |
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| Keywords: | Microcolony Bacteria enumeration |
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