| 诱导物对二氢嘧啶酶的作用 |
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| 引用本文： | 李隽 胡卓逸. 诱导物对二氢嘧啶酶的作用[J]. 中国药科大学学报, 1999, 30(3): 60-63 |
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| 作者姓名： | 李隽 胡卓逸 |
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| 作者单位： | 中国药科大学生物化学研究室!南京210009 |
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| 摘 要： | D对羟基苯甘氨酸可由二氢嘧啶酶水解DL对羟基苯海因制备得到。本文对Pseudomonas putida9801 菌所产生的二氢嘧啶酶诱导物进行了筛选。得出最佳的诱导物为甲硫乙基海因,最适诱导用量为0 .1 % 。与不加诱导物相比,采用分步诱导可以使酶活力增高近30 倍。
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| 关 键 词： | 诱导物 二氢嘧啶酶 D-对羟基苯甘氨酸 |
The Effect of Inducer on Enzyme Activity of Dihydropyri midinase |
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| Li Jun,Hu Zhuoyi Division of Biochemistry,China Pharmaceutical University,Nanjing. The Effect of Inducer on Enzyme Activity of Dihydropyri midinase[J]. Journal of China Pharmaceutical University, 1999, 30(3): 60-63 |
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| Authors: | Li Jun Hu Zhuoyi Division of Biochemistry China Pharmaceutical University Nanjing |
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| Affiliation: | Li Jun,Hu Zhuoyi Division of Biochemistry,China Pharmaceutical University,Nanjing 210009 |
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| Abstract: | The effect of several inducers on the activity of dihydropyrimidinase in grown %Pseudomonas putida% 9801 which biosynthesis %D p% hydroxyphenylglycine was studied. The best inducer is methylthioethylhydantoin. The optimum concentration of methylthioethylhydantoin is 0.1%. Compared enzyme activity of stepwise addition with enzyme activity without adding inducer, the activity of dihydropyrimidinase increases almost 30 times. |
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| Keywords: | Inducer Dihydropyrimidinase %D p% hydroxyphenylglycine |
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