中药消癃通闭对大鼠前列腺平滑肌一氧化氮合酶及上皮细胞凋亡的影响

目的：探讨中药消癃通闭对大鼠前列腺平滑肌中一氧化氮合酶（NOS）及上皮细胞凋亡的影响。方法：用还原辅酶Ⅱ组化染色结合形态学定量分析方法，检测各组大鼠前列腺中NOS神经含量；采用免疫组化原位末端标记染色法，检测大鼠前列腺上上细胞的凋亡百分率。结果：消癃通闭高剂量组在用药3周后，前列腺组织中NOS神经的长度密度（Lv)为0．113±0．023，与其他3组（消癃通闭低剂量组、保列治组及对照组）比较差异均有显著性（P＜0．01）。消癃通闭高剂量组及保列治组在实验第7天时，凋亡细胞百分率达到高峰（分别为9．27％、5．65％），第14天时稍减少，第21天时有所恢复。去势组大鼠在第7天时凋亡细胞即出现，到第14天时凋亡细胞百分率达到高峰，与其他组比较差异有显著性。结论：中药消癃通闭可增加模拟前列腺增生大鼠前列腺中NOS含量，在较短时间内促进大鼠前列腺上皮细胞凋亡。

Experimental Study on Effect of Xiaolong Tongbi on Nitric Oxide Synthase and Epithelial Cell Apoptosis in Prostate of Rats

OBJECTIVE: To explore the effect of Xiaolong Tongbi (XLTB), a Chinese herbal preparation, on nitric oxide synthase (NOS) containing nerve and epithelial cell apoptosis. METHODS: NOS containing nerve level of various groups prostate was determined by NADPH histochemical staining and morphological quantitative analysis, and the cell apoptosis rate in rats prostatic epithelium was tested immunohistochemically with TUNEL method. RESULTS: After 3 weeks treatment with high dose XLTB, the length density of NOS contained nerves in prostate tissue of rats was 0.113 +/- 0.023, which was significantly different to that in the other 3 groups (low dose XLTB, Finasteride and testosterone) respectively (P < 0.01). Cell apoptosis reached its peak at the day 7 of experiment in XLTB treated groups (9.27%), and Finasteride treated group (5.65%), which lowered slightly at day 14 and got some recovery at day 21. Castration induced cell apoptosis began at day 7 and reached its peak at day 14, which was significantly different from that in the other groups. CONCLUSION: XLTB could increase the NOS contained nerve level and accelerate the apoptosis of epithelial cell in prostate tissue of simulating benign prostatic hyperplasia rats.