针刺激对大鼠坐骨神经损伤后神经生长因子mRNA表达的影响

目的:探讨针刺对神经生长因子信使RNA(NGF mRNA)表达的影响,并且从这一角度分析评价电针频率的不同,及电针与手针对神经生长的影响。方法:78只雄性SD大鼠随机分为5组,治疗1组(n=18),疏波,电压2V,频率:F1:5Hz。治疗2组(n=18):疏波,电压2V,频率:F1:100Hz。治疗3组(n=18):手针。模型组(n=18):行坐骨神经损伤手术造模,不行治疗。对照组(n=6),正常成年大鼠。治疗组与模型组均行坐骨神经损伤手术造模,术后第2天开始给予电针及针刺治疗,电针正极接在近心端,负极接在远心端。分别夹在"环跳"、"足三里"处的针柄上,每次30min,每日2次。手针针刺相同穴位,每次30min,每日2次。术后1、2、6周取治疗组大鼠神经损伤部位远侧坐骨神经干0.6cm,应用原位杂交的方法和图像分析处理系统定量测定坐骨神经组织中NGF mRNA水平。结果:治疗组NGF mRNA表达明显增加,均高于模型组(均P<0.01);治疗1组NGF mRNA表达始终处于高水平,明显高于模型组(P<0.01)。结论:针刺激是促进周围神经损伤再生的重要手段,其中5Hz低频电针效果最佳。

Effects of acupuncture on the expression of nerve growth factor mRNA after sciatic nerve injury in rats

Objective To explore the effects of acupuncture on the expression of nerve growth factor (NGF) mRNA and actions of electroacupuncture with different frequencies and manual acupuncture to nerve growth. Methods Seventy-eight SD rats were randomly divided into 5 groups, electroacupuncture group with 5 Hz, electroacupuncture group with 100 Hz,manual-acupuncture group,model group with sciatic nerve injury,and control group. 30 min electroacupuncture or manual acupuncture at points "huantiao and zusanli" was performed twice daily one day after artificial injury of the sciatic nerve. NGF mRNA expressions in the sciatic nerve tissues were detected using in situ hybridization and the imaging analytic system on weeks 1, 2, and 6. Result The expressions of NGF mRNA were markedly higher in the acupuncture groups than in the model group, especially in the electroacupuncture group with 5 Hz. Conclusion Acupuncture is an important approach for accelerating regeneration of the impaired peripheral nerve and electroacupuncture with 5Hz achieves the optimal outcome.