12C6+重离子束照射人淋巴细胞蛋白质组分析

目的 探讨¹²C⁶⁺离子束照射对人淋巴细胞蛋白质组的影响。方法 将人淋巴细胞Peng-EBV按照射剂量分为0.1、0.5和2.0 Gy组，未照射为对照组。使用¹²C⁶⁺离子束进行照射，吸收剂量率为0.3~0.5 Gy/min。利用同位素标记的相对和绝对定量技术（iTRAQ）分析受照淋巴细胞的蛋白质表达，筛选出差异表达蛋白质。对差异表达的蛋白质进行基因本体（GO）分析、相互作用网络分析及通路分析。结果 在4组样品中共鉴定到5 158种蛋白质，与对照组相比，0.1 Gy组差异蛋白质有91种，0.5 Gy组有191种差异蛋白质，2.0 Gy组有68种差异蛋白质；3组共同差异表达的蛋白质有11种，其中，7种蛋白质表达上调，4种下调。对3个剂量组的差异表达蛋白质进行生物信息学分析显示，这些蛋白质主要与生物代谢及其调节过程、蛋白结合以及催化反应过程等有关，纤维黏连蛋白-1（FN1）和热休克蛋白1B（HSPA1B）是蛋白质相互作用网络的关键性节点。结论 不同剂量重离子照射诱导人淋巴细胞蛋白质组发生改变的机制不同，而且SZT2、FN1、HSPA1B蛋白质可能在重离子照射的损伤机制中发挥重要作用，有望成为重离子诱发辐射损伤的分子生物学标志。

Proteomics in human lymphoblastoid cells after 12C6+ ion irradiation

Objective To investigate the changes of proteomics in human lymphoblastoid cells after ¹²C⁶⁺ ion irradiation. Methods Human lymphoblastoid cells were irradiated with ¹²C⁶⁺ ion of 0, 0.1, 0.5 and 2.0 Gy at the dose rates of 0.3-0.5 Gy/min. Isobaric tags for relative and absolute quantitation (iTRAQ) was used to analyze the different protein expression in the irradiated cells. Gene ontology(GO), KEGG pathway and protein-protein interaction network analyses were performed. ResultsIn total identified 5 158 kinds of proteins, there were 91, 191, and 68 proteins being differentially expressed in the cells irradiated with 0.1, 0.5 and 2.0 Gy of ¹²C⁶⁺ ions, respectively, where 11 kinds of proteins were commonly expressed (7 up-regulated and 4 down-regulated) in these three groups compared with control. The analysis with bio-information softwares supposed that these differentially expressed proteins may play important roles in the metabolic process and regulation, protein binding and catalytic activity where fibronectin 1 (FN1) and heat shock protein 1B (HSPA1B) might be the key nodes. Conclusions Irradiation of ¹²C⁶⁺ ions can induce differentially expressed proteins in human lymphoblastoid cells. Analysis on functional roles of SZT2, FN1, and HSPA1B may provide new insight into further mechanistic investigations underlying the molecular biomarkers of ¹²C⁶⁺ ion irradiation.