Raf激酶抑制蛋白和p-ERK在糖尿病肾病大鼠肾组织中的表达及意义

 目的:探讨Raf激酶抑制蛋白(Raf kinase inhibitor protein,RKIP)和磷酸化细胞外信号调节激酶(phosphorylated extracellular signal-regulated kinase, p-ERK)在糖尿病肾病大鼠肾组织中的表达及意义。方法:40只Sprague-Dawley （SD）大鼠随机分为2组:正常对照组（N组）和糖尿病肾病组（M组）。苏木精-伊红（hematoxylin-eosin, HE）和过碘酸-雪夫（periodic acid-Schiff,PAS）染色观察肾组织病理改变，免疫组化方法检测各组肾组织RKIP和p-ERK1/2表达，Western blotting 检测各组RKIP和p-ERK1/2的表达。结果:M组大鼠随着病程的延长，血糖升高，出现蛋白尿，肾重增加，肾组织内p-ERK1/2明显增高，RKIP降低，与N组相比有显著差异(P<0.05)。结论: RKIP表达的减少及p-ERK的表达增加可能促进糖尿病肾病的进展。

Expression of Raf kinase inhibitor protein and p-ERK in renal tissues of diabetic rats

AIM: To investigate the expression of Raf kinase inhibitor protein (RKIP) and phosphorylated extracellular signal-regulated kinase (p-ERK) in the renal tissues of diabetic rats. METHODS: Forty SD rats were randomly divided into normal control group and diabetic nephropathy group. The pathological changes of the renal tissues were observed under microscope with HE and periodic acid-Schiff (PAS) staining. The expression of RKIP and p-ERK1/2 was detected by the methods of immunohistochemistry and Western blotting. RESULTS:  Following the extension of duration, the blood sugar of the rats in diabetic nephropathy group increased, with the appearance of proteinuria, increase in kidney weight, increase in renal p-ERK1/2 expression and decrease in RKIP expression as compared with control group. CONCLUSION: The development of diabetic nephropathy may be accelerated by the decrease in RKIP and the increase in p-ERK.