| UPLC法测定葛丹颗粒中葛根素的含量 |
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| 引用本文： | 许元宝.UPLC法测定葛丹颗粒中葛根素的含量[J].安徽医药,2013,17(2):210-211. |
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| 作者姓名： | 许元宝 |
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| 作者单位： | 安徽医科大学第一附属医院药剂科,国家中医药管理局中药化学三级实验室,安徽,合肥,230022 |
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| 摘 要： | 目的建立葛丹颗粒中葛根素含量的测定方法。方法采用超高效液相色谱法(UPLC)对方中君药葛根的主要活性成分葛根素进行含量测定,色谱条件为ACQUITY UPLC BEH C18(2.1 mm×50 mm,1.7μm)色谱柱,流动相:乙腈—0.1%磷酸(15∶85);流速:0.2 ml.min-1;柱温:30℃;检测波长:250 nm。结果葛根素在0.021 9～0.390 0 g.L-1范围内呈良好的线性关系,r=0.999 9,平均回收率为98.84%,RSD=0.71%。结论该法简单、快捷,适用于葛丹颗粒中葛根素的含量测定。
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| 关 键 词： | 葛丹颗粒 UPLC 葛根素 含量测定 |
UPLC determination of puerarin in gedan granule |
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| XU Yuan-bao.UPLC determination of puerarin in gedan granule[J].Anhui Medical and Pharmaceutical Journal,2013,17(2):210-211. |
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| Authors: | XU Yuan-bao |
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| Institution: | XU Yuan-bao (Department of Pharmacy, The First Affiliated Hospital of Anhui Medical University, Third-Grade Pharmaceutical Chemistry Laboratory of State Administration of Traditional Chinese Medicine,Hefei,Anhui 230022,China) |
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| Abstract: | Objective To establish a method for determination of puerarin in gedan granule. Methods The content of puerarin was de- termined by UPLC. The chromatographic conditions were as follows：ACQUITY UPLC BEH Cls （2.1 mm×50mm,1.7μm） used as analyrical column. The mobile phase consisted of a mixture of V （acetonitrile） ： V （ ortho-phosphoric acid） = 15： 85 and the flow rate was 0.2 mL min - l, with column temperature at 30℃, and UV detection at 250 nm. Results The puerarin was linear in the range of 0. 021 9 0. 390 0 g L- 1, r = 0.999 9. The average recovery rate was 98.84% （ RSD = 0.71% ）. Conclusion This method can be used to con- trol the quality of puerarin in gedan granule better. |
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| Keywords: | gedan granule UPLC puerarin determination |
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