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| 从转基因马铃薯中纯化的人白细胞介素-12的生物学活性分析 | |
| 引用本文： | 黄进,陈佳瑜,罗果,葛正龙. 从转基因马铃薯中纯化的人白细胞介素-12的生物学活性分析[J]. 遵义医学院学报, 2008, 31(6): 571-572 |
| 作者姓名： | 黄进 陈佳瑜 罗果 葛正龙 |
| 作者单位： | 1. 遵义医学院珠海校区,生物工程教研室,广东,珠海,519041 2. 遵义医学院,生物化学教研窒,贵州,遵义,563000 3. 遵义医学院,中心实验室,贵州,遵义,563000 |
| 基金项目： | 贵州省教育厅重点项目 |
| 摘 要： | 目的研究从转人白细胞介素-12（hIL-12）基因马铃薯中纯化的hIL-12的生物学活性。方法ELISA法测定纯化的hIL-12诱使外周血单个核细胞产生IFN-γ的量。结果纯化的hIL-12在体外能刺激PBMC产生IFN-γ，而野生对照组无作用。结论本实验室从转hIL-12基因马铃薯中纯化的hIL-12具有生物学活性。 |
| 关 键 词： | 人白细胞介素-12 纯化 生物学海性 转基因马铃薯 |
Bioactivity analysis of human interleukin 12 purified from transgenic potatoes |
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| HUANG Jin,CHEN Jia-yu,LUO Guo,GE Zheng-long. Bioactivity analysis of human interleukin 12 purified from transgenic potatoes[J]. Acta Academiae Medicine Zunyi, 2008, 31(6): 571-572 | |
| Authors: | HUANG Jin CHEN Jia-yu LUO Guo GE Zheng-long |
| Affiliation: | HUANG Jin1,CHEN Jia-yu2,LUO Guo3,GE Zheng-long*(1.Department of Bioengineering,Zunyi Medical College Zhuhai Campus,Guangdong Zhuhai 519041,China,2.Department of Biochemistry,Zunyi Medical College,Guizhou Zunyi 563000,3.The Central Laboratory,Zunyi Medied College,Guizhou Zunyi 563003,China) |
| Abstract: | Objective To investigate the bioactivity of human interleukin 12 （hIL-12） purified from transgenic potatoes breeded by our research group. Methods ELISA was used to assess IFN-γ secretion of Peripheral blood mono-nuelear eells（PBMC） indueed by purified hIL-12. Results The purified hIL-12 was able to induce PBMC to secrete IFN-γ in vitro ,while wild potatoes was inactivity. Conclusions hIL-12 purified from transgenie potatoes breeded by our research group is bioaetive . |
| Keywords: | human interleukin 12 purification bioactivity transgenic potato |
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