北京市部分男男同性恋人群中HIV-1分子流行病学研究

目的了解北京市部分男男同性恋(men who have sex with men,MSM)人群HIV-1的分子流行情况,监测该地区人群HIV毒株的最新流行情况。方法提取17例男男同性恋者HIV-1抗体阳性样本全血基因组DNA,直接进行巢式PCR扩增HIVenv基因C2-V5区及gag基因P17-P24区,对PCR产物进行核苷酸序列测定和分析,确定基因亚型。结果17例患者中,共存在CRF01-AE、B亚型和CRF07-BC3种亚型,其中CRF01-AE9例(52.94%),B亚型5例(29.41%),CRF07-BC3例(17.65%)。结论小样本量的流行病学调查显示,北京地区部分男男同性恋人群中主要存在CRF01-AE、B和CRF07-BC3种亚型;CRF01-AE已取代B亚型而成为北京市MSM人群中HIV主要流行亚型,CRF07-BC重组亚型在北京市MSM人群中增长迅速。

Molecular Epidemiologic Study on Human Immunodeficiency Virus Type 1 Infection in Partial Men Who have Sex with Men(MSM) in Beijing

Objective To investigate the molecular epidemiology of human immunodeficiency virus 1(HIV-1) infection among men who have sex with men(MSM) in Beijing and survey the up to date HIV-1 prevalence in this region. Methods Seventeen anti-HIV-1 positive samples of MSMs from 2007 2008 were collected, the genomic DNA were isolated directly from the whole blood, HIV-1 gag/env genes were amplified by nested PCR from the genomie of MSMs. The c2-v5 region of env gene and p17-p24 region of gag gene were sequenced for analyzing the subtype directly. Phylogenetic analyses were performed based on the nucleotide sequence data. Results There were three HIV-1 strains including CRF01-AE, B and CRF07-BC among seventeen MSMs in Beijing, genotyping showed that 52.94%(9/17) were CRF01-AE, 29.41%(5/17) were B and 17.65%(3/17) were CRF07-BC. Conclusion There are three HIV-1 subtypes among MSMs in Beijing from the small samples epidemiologic survey, and the CRF01-AE has replaced the B and became the most prevalent HIV-1 subtype in Beijing, and the CRF07-BC is increasing fast among MSMs in Beijing recently.