| 苦参素诱导人胃癌MGC-803细胞凋亡的体内外实验研究 |
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| 引用本文： | 朱艳琴,刘粉霞,徐玉芳.苦参素诱导人胃癌MGC-803细胞凋亡的体内外实验研究[J].中国药学杂志,2011,46(1):28-30. |
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| 作者姓名： | 朱艳琴 刘粉霞 徐玉芳 |
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| 作者单位： | 1. 河南中医学院基础医学院，郑州 450008；2. 河南中医学院第二附属医院，郑州 450008 |
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| 摘 要： | 目的 探讨苦参素体内外诱导人胃癌MGC-803细胞凋亡的作用及机制.方法 运用流式细胞术检测细胞凋亡率及凋亡相关基因蛋白Bel-2和Bax的表达水平;运用TUNEL法观察裸鼠皮下移植瘤细胞凋亡情况.结果 经体内外实验研究结果表明,苦参素能促进人胃癌MGC-803细胞凋亡,其细胞凋亡率与阴性对照组相比差异具有统计学意义(...
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| 关 键 词： | 苦参素 流式细胞术 TUNEL 人胃癌MGC-803细胞 凋亡 |
| 收稿时间： | 2011-11-11; |
Experimental Study on Oxymatrine Inducing Apoptosis of Human Carcinoma Cells MGC-803 in Vivo and in Vitro |
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| ZHU Yan-qin,LIU Fen-xia,XU Yu-fang.Experimental Study on Oxymatrine Inducing Apoptosis of Human Carcinoma Cells MGC-803 in Vivo and in Vitro[J].Chinese Pharmaceutical Journal,2011,46(1):28-30. |
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| Authors: | ZHU Yan-qin LIU Fen-xia XU Yu-fang |
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| Institution: | 1.College of Basic Medical Sciences, Henan College of Traditional Chinese Medicine, Zhengzhou 450008, China； 2. Second Affiliated Hospital，Henan College of Traditional Chinese Medicine，Zhengzhou 450008，China |
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| Abstract: | OBJECTIVE To explore effect of oxymatrine on MGC-803 cell apoptosis and its possible mechanism in vivo and in vitro. METHODS Apoptosis rate and the expression level of Bcl-2 and Bax were determined with flow cytometry. Subcutaneous transplantation tumor cell apoptosis was observed in nude mouse with TUNEL. RESULTS The study in vivo and in vitro indicated that oxymatrine promoted MGC-803 cell apoptosis. The index of apoptosis was significantly different (P﹤0.05 compared with the control group. The expression of Bcl-2 was reduced and the expression of Bax was increased remarkably ( P﹤0.05. CONCLUSION Oxymatrine can induce MGC-803 cell apoptosis in vivo and in vitro. The mechanism was related to up-regulating expression of Bax and down-regulating expression of Bcl-2. |
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| Keywords: | oxymatrine flow cytomete TUNEL human carcinoma cells MGC-803 apoptosis |
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