高效液相色谱-质谱法检测Wistar大鼠主动脉6-keto-PGF_（1α）的生成

目的：建立大鼠主动脉6-keto-PGF1α的高效液相色谱-质谱（HPLC-MS）检测方法,以反映前列环素（PGI2）的产生水平。方法：建立用HPLC-MS同时检测环氧化酶（COX）代谢通路的5种前列腺素类产物的方法和Wistar大鼠主动脉产生的6-keto-PGF1α水平。结果：HPLC-MS检测法能同时检测COX代谢通路的5种PGI2产物和大鼠主动脉产生的6-keto-PGF1α;加入内皮受体激动剂乙酰胆碱后6-keto-PGF1α的生成明显升高。结论：成功建立用HPLC-MS检测Wistar大鼠主动脉所生成6-keto-PGF1α的方法。

Determination of 6-keto-PGF1α in Rat Aortas with High Performance Liquid Chromatography-Mass Spectrometry

Objective：To detect the synthesis level of prostacyclin（PGI2）in rat aortas with the established method of high performance liquid chromatography-mass spectrometry（HPLC-MS）.Methods： HPLC-MS was established to analyze the five kinds of prostaglandin productions derived from cyclooxygenase（COX）mediated metabolism.The PGI2 metabolite 6-keto-PGF1α of aortas isolated from Wistar rats was analyzed.Results： HPLC-MS could be applied to detect the five prostaglandin productions derived from the COX-mediating pathway.The PGI2 metabolite 6-keto-PGF1α produced by Wistar rat aortas was well determined by HPLC-MS.The amount of 6-keto-PGF1α was obviously augmented when stimulated with the endothelial receptor agonist acetylcholine.Conclusion： The method of HPLC-MS is well established to detect 6-keto-PGF1α produced by aortas from Wistar rats.