高效液相色谱法测定疲王按摩乳中阿魏酸的含量 |
| |
引用本文: | 涂禾,;梁栗,;侯世祥,;冯光富.高效液相色谱法测定疲王按摩乳中阿魏酸的含量[J].华西医学,2014(9):1697-1700. |
| |
作者姓名: | 涂禾 ;梁栗 ;侯世祥 ;冯光富 |
| |
作者单位: | [1]四川省骨科医院药剂科,成都610041; [2]四川大学华西药学院,成都610041; |
| |
基金项目: | 国家“十五”科技攻关项目(2002BA904803) |
| |
摘 要: | 目的建立疲王按摩乳中阿魏酸的含量测定方法。方法采用高效液相色谱法,色谱柱为Diamonsil C18(150mm×4.6mm,5μm,北京迪马科技有限公司);流动相为甲醇.2%冰醋酸(32:68);柱温为30℃;检测波长为320nm。结果阿魏酸在4.55~91.00μg/mL范围内呈良好的线性关系(R2=0.9998),其平均加样回收率分别为98.78%(相对标准偏差为2.668%)。结论该测定方法简便、快速、重现性好,可作为疲王按摩乳的质量控制方法。
|
关 键 词: | 疲王按摩乳 高效液相色谱法 阿魏酸 含量测定 |
The Content Determination of Ferulic Acid in Piwang Massage Lotion by High Performance Liquid Chromatography |
| |
Institution: | TU He, LIANG Zi, HOU Shi-xiang, FENG Guang-fu. (1. Department of Pharmacy, Sichuan Orthopedic Hospital, Chengdu, Sichuan 610041, P. R. China; 2. West China School of Pharmacy, Sichuan University, Chengdu, Sichuan 610041, P. R. China) |
| |
Abstract: | Objective To establish a method for content determination of ferulic acid in Piwang massage lotion. Methods High performance liquid chromatography was used. The analysis was carried on Diamonsil C18 (150 mm× 4.6 mm, 5 μm, Dimma Science and Technology Co. Ltd.) column with mobile phase consisted of methanol-2% acetic acid (32 : 68). The column temperature was 30 ℃ . The detection wavelength was 320 nm. Results The responses of ferulic acid liner in range of 4.55-91.00 μg/mL (R2=0.999 9). The average recovery of ferulic acid was 98.78 % (relative standard deviation is 2.668 %). Conclusions The method is simple, rapid and good repeatability. It can be used for quality control of Piwang massage lotion. |
| |
Keywords: | Piwang massage lotion High performance liquid chromatography Ferulic acid Content determination |
本文献已被 维普 等数据库收录! |
|