| 高效液相色谱法测定疲王按摩乳中阿魏酸的含量 |
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| 引用本文： | 涂禾,;梁栗,;侯世祥,;冯光富.高效液相色谱法测定疲王按摩乳中阿魏酸的含量[J].华西医学,2014(9):1697-1700. |
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| 作者姓名： | 涂禾 ;梁栗 ;侯世祥 ;冯光富 |
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| 作者单位： | [1]四川省骨科医院药剂科,成都610041; [2]四川大学华西药学院,成都610041; |
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| 基金项目： | 国家“十五”科技攻关项目（2002BA904803） |
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| 摘 要： | 目的建立疲王按摩乳中阿魏酸的含量测定方法。方法采用高效液相色谱法，色谱柱为Diamonsil C18（150mm×4．6mm，5μm，北京迪马科技有限公司）；流动相为甲醇．2％冰醋酸（32：68）；柱温为30℃；检测波长为320nm。结果阿魏酸在4．55～91．00μg／mL范围内呈良好的线性关系（R2=0．9998），其平均加样回收率分别为98．78％（相对标准偏差为2．668％）。结论该测定方法简便、快速、重现性好，可作为疲王按摩乳的质量控制方法。
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| 关 键 词： | 疲王按摩乳 高效液相色谱法 阿魏酸 含量测定 |
The Content Determination of Ferulic Acid in Piwang Massage Lotion by High Performance Liquid Chromatography |
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| Institution: | TU He, LIANG Zi, HOU Shi-xiang, FENG Guang-fu. (1. Department of Pharmacy, Sichuan Orthopedic Hospital, Chengdu, Sichuan 610041, P. R. China; 2. West China School of Pharmacy, Sichuan University, Chengdu, Sichuan 610041, P. R. China) |
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| Abstract: | Objective To establish a method for content determination of ferulic acid in Piwang massage lotion. Methods High performance liquid chromatography was used. The analysis was carried on Diamonsil C18 （150 mm× 4.6 mm, 5 μm, Dimma Science and Technology Co. Ltd.） column with mobile phase consisted of methanol-2% acetic acid （32 ： 68）. The column temperature was 30 ℃ . The detection wavelength was 320 nm. Results The responses of ferulic acid liner in range of 4.55-91.00 μg/mL （R2=0.999 9）. The average recovery of ferulic acid was 98.78 % （relative standard deviation is 2.668 %）. Conclusions The method is simple, rapid and good repeatability. It can be used for quality control of Piwang massage lotion. |
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| Keywords: | Piwang massage lotion High performance liquid chromatography Ferulic acid Content determination |
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