| HPLC测定白金丸中吉马酮的含量 |
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| 引用本文： | 姜雪强,;陈银华,;李志浩. HPLC测定白金丸中吉马酮的含量[J]. 郧阳医学院学报, 2014, 0(6): 582-584 |
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| 作者姓名： | 姜雪强, 陈银华, 李志浩 |
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| 作者单位： | [1]湖北医药学院附属东风医院,武当特色中药研究湖北省重点实验室; [2]十堰市食品药品监督检验所,湖北十堰442008 |
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| 基金项目： | 湖北省教育厅科学技术研究指导项目（B2014050） |
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| 摘 要： | 目的:建立测定白金丸中吉马酮含量的高效液相色谱方法。方法:采用Fortis-C18柱(4.6 mm×250 mm,5μm),以乙腈∶水(73∶27)为流动相,流速1.0 m L·min-1,柱温30℃,检测波长220 nm。结果:吉马酮在0.00840.1680 mg/m L浓度范围内有良好线性关系(r=0.9998),平均加样回收率为99.0%,RSD为1.66%。结论:本法简便、快速、准确、重复性好,可用于白金丸中吉马酮的含量测定。
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| 关 键 词： | 白金丸 吉马酮 高效液相色谱法 |
Determination of Germacrone in Baijin Wan by HPLC |
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| Affiliation: | JIANG Xue-qiang, CHEN Yin-hua, LI Zhi-hao (IDongfeng Hospital of Hubei University of Medicine, Hubei Key Laboratory of Wndang Local Chinese Medicine Research ; 2 Shiyan Institute for Food and Drug Control, Shiyan,Hubei 442000, China) |
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| Abstract: | Objective To establish a HPLC method for the determination of germacrone in Baijin Wan. Methods The HPLC system consisted of the Fortis-C18 （4.6 mm ×250 mm,5 μm）column,the mobile phase consisted of HCN-H2O（73： 27）, the flow rate was 1.0 mL · min^-1 ,the column temperature was 30 0C ,the UV detector was set at 220 nm. Results The linear responsive range was from O. 0084 to 0. 1680 mg/mL（ r =0. 9998）. The average recovery of germacrone was 99.0% ,and RSD was 1.66%. Conclusion The method is simple,rapid,accurate and repeatable. It can be applied for determination of germacrone in Baijin Wan. |
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| Keywords: | Baijin Wan Germacrone HPLC |
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