3种血清总胆红素测定方法的比较

目的对钒酸氧化法、改良J-G法、胆红素氧化酶法进行方法学比较,选择胆红素测定的最好方法。方法用钒酸氧化法、改良J-G法和胆红素氧化酶法测定血清中的总胆红素,按NCCLC评价方案系统评价其重复性、回收率、线形范围及溶血、脂血对胆红素测定的干扰影响,然后对测定结果进行比较。结果钒酸氧化法与改良J-G法、胆红素氧化酶法有良好的相关性;其回收率均在95.0%~105.0%;其线形范围分别是:0~678μmol/L,0~634μmol/L,0~575μmol/L。三酰甘油≤8.0 mmol/L、血红蛋白≤6.0 mmol/L对钒酸氧化法无明显干扰。结论钒酸氧化法测定胆红素准确、灵敏、稳定,线形范围宽,是测定胆红素的理想方法。

Comparison of three technology to determine serum total bilirubin

Objective It is to select the best method to determine bilirubin through technology comparison between vanadic acid oxidation method,improved J-G method and Bilirubin Oxidase method.Methods Serum total bilirubin was determined by vanadic acid oxidation method,improved J-G method and Bilirubin Oxidase method.Reproducibility,recovery rate,filate range,interference of haematolysis and lipaemia were evaluated by NCCLC system,and then the results were compared.Results There were favourable dependability between vanadic acid oxidation method,improved J-G method and Bilirubin Oxidase method.Recovery rate were all between 95.0%～105.0%;filate range was 0～678 μmol/L,0～634 μmol/L,0～575 μmol/L respectively.There were not obviously interference to vanadic acid oxidation method when TG≤8.0 mmol/L,HB≤6.0 mmol/L.Conclusion Vanadic acid oxidation method is accurate,sensitive,stable and has wide filate range.So it is ideal method to determine bilirubin.