HLA class I and II typing of the patients with Behçet's disease in Saudi Arabia |
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Authors: | K Yabuki S Ohno N Mizuki H Ando KF Tabbara K Goto E Nomura S Nakamura N Ito M Ota Y Katsuyama H Inoko |
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Institution: | Department of Genetic Information, Tokai University School of Medicine, Isehara, Kanagawa, Japan. |
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Abstract: | Thirteen Saudi Arabian patients with Beh?et's disease (BD) were typed for HLA-A and -B alleles by the conventional serologic typing and for HLA-DRB1, -DQB1 and -DPB1 alleles by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. As a result, the phenotype frequency of the B51 antigen was significantly increased in the patient group as compared to the ethnically matched control group (76.9% in patients vs. 22.20% in controls), but no significant difference was observed in HLA-A, -DRB1, -DQB1 or -DPB1 alleles between the patients and controls, as previously observed in Japanese BD. Further, by HLA-B51 allelic genotyping performed by the polymerase chain reaction-sequence specific primers (PCR-SSP) method, all of the B51-positive patients and controls were found to carry one particular allele, B*5101, except one patient with B*5108. |
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Keywords: | Behçet's disease B51 genotyping HLA PCR-SSP Saudi Arabian |
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