肿瘤坏死因子基因和蛋白及受体在子宫内膜组织的表达

目的探讨子宫内膜癌组织肿瘤坏死因子（ＴＮＦ）基因和蛋白及受体的表达及意义。②方法分别采用非同位素原位杂交方法、免疫组化方法对４８例子宫内膜癌组织ＴＮＦｍＲＮＡ，ＴＮＦ蛋白及ＴＮＦ受体（ＴＮＦＲ）的表达进行检测。③结果子宫内膜癌组织中１６例ＴＮＦｍＲＮＡ呈阳性表达，均为间质的单个核细胞和平滑肌细胞，腺癌细胞无阳性表达，对照组未见阳性表达。１５例病人ＴＮＦ蛋白呈阳性表达，２５例ＴＮＦＲ呈阳性表达，对照组子宫内膜腺体也有ＴＮＦＲ阳性表达，两组比较差异无显著性（χ２＝２．４５，Ｐ＞０．０５）。ＴＮＦｍＲＮＡ与ＴＮＦ蛋白表达存在不一致性，ＴＮＦ与ＴＮＦＲ表达与组织学分化及肌层浸润深度差异均无显著性（χ２＝２．１７，０．８１，Ｐ＞０．０５）；ＴＮＦＲ蛋白表达明显高于ＴＮＦ，两者比较差异有极显著性（χ２＝４．２９，Ｐ＜０．０５）；ＴＮＦＲ在正常内膜表达与子宫内膜癌表达差异无显著性（χ２＝２．４５，Ｐ＞０．０５）。④结论子宫内膜癌间质细胞能够表达ＴＮＦｍＲＮＡ，并产生ＴＮＦ蛋白。

EXPRESSIONS OF GENE, PROTEIN AND RECEPTOR PROTEIN OF TUMOR NECROSIS FACTOR IN ENDOMETRIAL TISSUES

\ Objective \ To observe the expressions and distributions of tumor necrosis factor (TNF) mRNA, TNF protien and TNF receptor(TNFR) in endometrial carcinomas and investigate their clinicopathological significance. \ Methods \ The expressions of TNF mRNA, TNF protein and TNFR in 48 cases of endometrial carcinoma were studied by in situ hybridization and immunohistochemical techniques.\ Results \ TNF mRNA expression was detected in the stroma such as mononuclear cells and smooth muscles in 16 cases of endometrial carcinoma. There was no TNF mRNA expression in the carcinoma cells and controls. TNF protein was positive in 15 cases of endometrial carcinoma. TNFR expression was detected both in endometrial carcinomas(25 cases) and controls(18 cases), the differences was not significant (2=2.45, P>0.05). The expressions of TNF and TNFR in cases of different histologic grades and depth of invasion were not significantly different(2=2.17, P>0.05). TNFR expression in endometrial carcinoma was higher than that of TNF(2=4.29, P<0.05). The expression of TNFR in normal endometrium and endometrial carcinoma was not significantly defferent(2=2.45, P>0.05).\ Conclusion \ The stroma cells in endometrial carcinoma were capable of expressing TNF mRNA and producing TNF protein.