Impact of APRF+ in Combination with Autogenous Fibroblasts on Release Growth Factors,Collagen, and Proliferation and Migration of Gingival Fibroblasts: An In Vitro Study |
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| Authors: | Barbara Stercza&#x;a Agnieszka Chwi&#x;kowska Urszula Szwedowicz Magdalena Kobielarz Bart&#x;omiej Chwi&#x;kowski Marzena Dominiak |
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| Institution: | 1.Dental Surgery Department, Wroclaw Medical University, 50-425 Wroclaw, Poland;2.Department of Molecular and Cell Biology, Wroclaw Medical University, 50-556 Wroclaw, Poland;3.Department of Mechanics, Materials and Biomedical Engineering, Wroclaw University of Science and Technology, 50-371 Wroclaw, Poland;4.Department of Applied Mathematics, Faculty of Pure and Applied Mathematics, Wroclaw University of Science and Technology, 50-370 Wroclaw, Poland; |
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| Abstract: | The present study aimed to compare the action of advanced platelet-rich fibrin (A-PRF+) alone with the action of A-PRF+ combined with autologous gingival fibroblasts. The components released from A-PRF+ conditioned with autogenous fibroblasts that were quantified in the study were fibroblast growth factor (FGF), vascular endothelial growth factor (VEGF), trans-forming growth factor-beta1 and 2 (TGFβ1 and TGFβ2), and soluble collagen. A-PRF+ combined with fibroblasts demonstrated significantly higher values of released VEGF at every time point and, after 7 days, significantly higher values of released TGFβ2. A viability test after 72 h showed a significant increase in proliferation fibroblasts after exposition to the factors released from A-PRF+ combined with fibroblasts. Similarly, the degree of wound closure after 48 h was significantly higher for the factors released from A-RRF+ alone and the factors released from A-RRF+ combined with fibroblasts. These results imply that platelet-rich fibrin (PRF) enhanced with fibroblasts can be an alternative method of connective tissue transplantation. |
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| Keywords: | A-PRF+ fibroblast culture wound healing VEGF TGFβ 2 |
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