ibrolipim 对糖尿病小型猪肝脏PKBβ/Akt2表达的影响

目的 采用高脂高糖高胆固醇饮食喂养的方法建立广西巴马小型猪糖尿病动物模型.研究合成化合物ibrolipim(NO-1886)对肝脏PKBβ/Akt2表达变化的影响,探讨ibrolipim改善胰岛素抵抗、治疗糖尿病的可能机制.方法 15头雄性广西巴马小型猪按体重随机分为3组:正常对照组(n=5)饲以正常饲料;高脂高糖高胆固醇组(n=5)饲以高脂高糖高胆固醇饲料;加药组(n=5),HFSCD饲料中加入1.0%NO-1886.每月末测定血糖、甘油三酯、胰岛素和口服葡萄糖耐量实验.第5个月末取部分肝脏组织用western blotting和免疫组织化学方法检测Akt2蛋白的表达.结果 CD组血糖、甘油三酯、胰岛素水平正常,葡萄糖耐量实验(OGTT)血糖清除和胰岛索分泌有效、及时;与CD组相比较,HFSCD组血糖和甘油三酯胰岛素水平明显升高,糖耐量减低;HFSCD NO-1886组与HFSCD组相比,血糖、甘油三酯和胰岛素水平明显降低,OGTT葡萄糖清除和胰岛素分泌近似CD组.CD组肝脏Akt2蛋白表达鼍较高;HFSCD组Akt2表达量减少(P<0.05);HFSCD 1%NO-1886组Akt2蛋白表达较HFSCD明显增高(P<0.05)但低于CD组.结论 饮食诱导的糖尿病小型猪肝脏组织Akt2表达降低;NO-1886能够上调肝脏Akt2蛋白的表达.改善胰岛素抵抗.

Effect of Lipoprotoin Lipase Activator ibrolipim on Expression of Protein Klnase - B in Hepatic of Diabetic Miniature Pigs

Objective To investigate the effect of lipoprotein lipase (LPL) activator NO-1886 on the protein expression of the protein kinase-B (PKB/Akt) in liver of diabetic minipigs induced by high-fat/high-sucrose/high-cholesterol diet.Methods Fifteen Guangxi Bama minipigs were randomized into three groups: CD group (n=5), HFSCD group (n=5), and HFSCD+NO-1886 group (n=5). plasma glucose, insulin, triglyceride and oral glucose tolerance test(OGTT) were measured each month following overnight fasting.The pigs were sac...