自来水及水源水有机提取物类雌激素活性研究

为了检测自来水及水源水有机提取物的类雌激素活性 ,应用环境雌激素基因重组酵母、细胞增殖和子宫增重等三项短期生物学实验进行测定。结果发现 :水样有机提取物在相当于原水量 50ml时两项体外实验均检出雌激素活性 ,原水量 2 50ml时细胞增殖效应达到最大 ,以后随原水量增大细胞增殖效应逐步减弱。原水量 12 50ml时酵母菌β -半乳糖苷酶活性最强 ,以后随原水量增大 β -半乳糖苷酶活性逐步减弱。水厂水源水有机提取物低、中剂量组受试小鼠子宫湿重增加 ,与对照组比较有统计学意义 ,高剂量组湿重减少。提示水源水有机提取物具有类雌激素活性。采用这三项实验组成的生物学检测程序具有一定的灵敏度 ,且较快速、方便。

Study on the estrogenic effect of the organic extracts from tap water and source water

In order to study the estrogenic effects of the organi c extracts from tap water and source water, two in vitro assay and one in vivo s hort-term assay were performed to detect the estrogen-like activity of the com ponent of water. The results showed that at the concentration of 50ml relevant t o real water, the recombinant yeast assay and MCF-7 cell proliferation assay co uld both detected the estrogen-like activity and with increase of concentration , the component exhibited an increasing estrogenic effects. The dose inducing t he maximum effect in cell proliferation assay was 250ml and in recombinant yeast assay was 1250ml. Exceed this dose with increasing concentration, the estrogeni c effects of component decreased both in two assay. In uterrtropic assay, doses of 10L/kg and 50L/kg increased the weight of uterus of 18 day ICR female mice si gnificantly. All above findings indicated that organic extracts from source wate r exhibited the estrogenic effects. The series of 3 bioassay process displayed s ensitivity and practicability.