Influence of Mitragyna Ciliata (MYTA) on the Microsomal Activity of ATPase Na+/K+ Dependent Extract on a Rabbit Heart |
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| Authors: | Alain dit Philippe Bidie Ernest Koffi Jean-David N'Guessan Allico Joseph Djaman Fr��d��rique Guede-Guina |
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| Affiliation: | 1Laboratoire de pharmacodynamie Biochimique ; UFR Biosciences Université de Cocody, 22 BP 582 Abidjan 22 Côte d''Ivoire;2Laboratoire de Biochimie et de Sciences des Aliments ; UFR Biosciences Université de Cocody, 22 BP 582 Abidjan 22 Côte d''Ivoire |
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| Abstract: | Mitragyna ciliata (MYTA) (Rubiaceae) inhibits plasmodia activity. MYTA induces a cardiotonicity of the digitalic type on rat''s isolated heart. In this work we studied the effect of MYTA on microsomal Na+/K+ dependant ATPase (Na+, K+ ATPase) extracted from the heart of a rabbit since digitalics inhibit Na+, K+ ATPase. Our results revealed that the Na+/K+ ATPase has an optimum pH of 7.4 and temperature of 37°C respectively. There is a linear relationship between the organic phosphate formed and the incubation time over 25 mins incubation period. The ATP hydrolysis rate in the presence of MYTA was 0.775 µM/min. LINEWEAVER and BURK plots showed that MYTA did not alter KM (1.31 mM) but decreased VMAX. This study shows that MYTA exerts a non-competitive inhibition on the microsomal Na+/K+ ATPase extracted from rabbit heart with a Ci50 of 48 µg / ml. We conclude that the mechanism of action of MYTA is linked to the inhibition of the Na+/K+ ATPase like cardiotonics of the digitalic type. |
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| Keywords: | Mitragyna ciliata ATPase Na+/K+ inhibitors of ATPase Na+/K+ |
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