ATP对人不死化成纤维细胞增殖及其细胞膜蛋白表达的影响

目的探讨ATP对不死化人成纤维细胞增殖及其细胞膜蛋白表达的影响。方法将正常人TIG-7和0UMS-36细胞株，不死化人KMST-6和SUSM-1细胞株在不同浓度的ATP，ADP，AMP条件下分别进行24和96h的常规细胞培养，观察存活细胞数目，DNA的合成和

Effect of ATP on proliferation and membrane protein expression in immortalized hmnan fibroblasts

AIM: To investigate the effect of ATP on proliferation and membrane protein expression in immortalized human fibroblasts. METHODS: Applying the normal human fibroblast cell line TIG-7 and OUMS-36, the immortalized human fibroblast cell line KMST-6 and SUSM-1 to culture for 24 hours and 96 hours with the different concentrations of ATP,ADP and AMP, respectively. The number of cell alive, DNA synthesis and the expression of 32P]-ATP labeled proteins in the membrane fraction were also observed. RESULTS: After 96 hours culture, the proliferation inhibition in immortalized cells exposed to 0.4mmol/L ATP was 77%, while that of normal OUMS-36 cells treated with the same concentration of ATP was 41%; the DNA synthesis of the immortalized cells exposed to 0.4mmol/L ATP was greater suppressed than that of normal OUMS-36 cells. ADP was the only reagent tested that reduced the number of cells significantly(P<0.01).When the immortalized cells exposed to ADP,AMP, adenosine, and phosphoric acid. In the presence of 1 mmol/L ATP, many KMST-6 cells died, but normal OUMS-36 cells did not. Compared with normal cells, the expresson of 32P]-ATP labeled 30 kD, 31 kD, 33 kD and 40 kD proteins were markedly higher in the immortalized cells. CONCLUSION: ATP inhibited the proliferation and increased membrane protein expression in immortalized human fibroblasts.