Immunolocalization and heart levels of GRP94 in the mouse during post-implantation development |
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Authors: | J A Barnes and Ida W Smoak |
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Institution: | (1) College of Veterinary Medicine, North Carolina State University, 4700 Hillsborough Street, Raleigh, NC 27606, USA Fax: (919)829-4465; e-mail: smoak@sn1.cvm.ncsu.edu, US |
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Abstract: | Glucose-regulated proteins (GRPs), which belong to the highly conserved family of stress proteins, are resident to the endoplasmic
reticulum and function as molecular chaperones. Heat shock proteins have been shown to be developmentally regulated, but little
work has been done to investigate the expression of GRPs during embryogenesis. Therefore, this study examined the distribution
of GRP94 within mouse embryos during the period of organogenesis and characterized levels of GRP94 within the developing heart
during organogenesis and late fetal stages. Our results demonstrate that the GRP94 protein is constitutively expressed within
mouse embryos during early stages of organogenesis and is localized particularly within the developing heart, neuroepithelium,
and surface ectoderm tissues. Positive staining for GRP94 remains within developing heart tissues throughout organogenesis
and is found primarily within the atrial and ventricular myocardial cells. Western blot analysis of GRP94 expression demonstrates
a significantly higher level of GRP94 in embryonic hearts during early stages of organogenesis than in later stages of organogenesis
or the fetal period. These results demonstrate that the stress protein GRP94 is constitutively expressed within specific tissues
during post-implantation mouse development and suggest that GRPs may play an important role in the process of myocardial cell
differentiation and heart development.
Accepted: 9 June 1997 |
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Keywords: | Stress protein Western blotting Mouse embryo Cardiovascular system Immunohistochemistry |
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