| 慢性乙肝患者血清及外周血单个核细胞中HBV DNA的研究 |
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| 引用本文： | 王豪,杜绍财,陈苹,陶其敏.慢性乙肝患者血清及外周血单个核细胞中HBV DNA的研究[J].北京大学学报(医学版),1993(2). |
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| 作者姓名： | 王豪 杜绍财 陈苹 陶其敏 |
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| 作者单位： | 北京医科大学肝病研究所
(王豪，杜绍财，陈苹)，北京医科大学肝病研究所(陶其敏) |
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| 摘 要： | 利用国产生物素交联光敏补骨酯素(Biotin—Psoralen)标记核酸探针,建立了外周血单个核细胞(PBMC)的原位杂交方法,同时应用聚合酶链反应技术(PCR)检测了血清中的HBV DNA,结合PBMC的原位杂交结果,对HBV DNA在PBMC中存在的状况和意义进行了初步探讨。
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| 关 键 词： | 原位杂交 PCR HBV DNA |
THE STUDY ON HBV DNA IN SERUM AND PBMC IN PATIENTS WITH CHRONIC HBV INFECTION |
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| Wang Hao Institute of Hepatology.THE STUDY ON HBV DNA IN SERUM AND PBMC IN PATIENTS WITH CHRONIC HBV INFECTION[J].Journal of Peking University:Health Sciences,1993(2). |
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| Authors: | Wang Hao Institute of Hepatology |
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| Institution: | Wang Hao Institute of Hepatology |
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| Abstract: | The method of in situ hybridisization on peripheral blood mononuclear cells(PBMC)was estab-lished using biotin linked photo-psoralen HBV DNA probe.HBV DNA in serum was detected simulta-neously with technology of polymerase chain reaction(PCR).The circumstance and significance ofHBV DNA exsting in PMBC were discussed. |
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| Keywords: | In situ hybridisation PCR HBV DNA |
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