Estrogen target cells during the early stage of medullary bone osteogenesis: Immunohistochemical detection of estrogen receptors in osteogenic cells of estrogen-treated male Japanese quail |
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Authors: | Tomoo Ohashi Seiji Kusuhara Kazuo Ishida |
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Institution: | (1) Graduate School of Science and Technology, Niigata University, 950-21 Niigata, Japan;(2) Department of Animal Secience, Faculty of Agriculture, Niigata University, 950-21 Niigata, Japan |
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Abstract: | Summary The localization of estrogen receptors (ER) in osteogenic cells during the early stage of medullary bone osteogenesis was
studied immunohistochemically in the femurs of estrogen-treated male Japanese quail. Alkaline phosphatase (ALP) activity was
used as a marker for osteogenic cells. ER immunostaining was observed in the nuclei of weak ALP-positive bone lining cells
on the endosteal bone surface of nontreated birds. After 24 hours of estrogen treatment, nuclear immunostaining was detected
in ALP-positive preosteoblasts on the endosteal bone surface. After 48 hours, the medullary bone appeared to some degree along
the endosteal surface. ER immunostaining was observed in the nuclei of ALP-positive osteoblasts on the medullary bone surface.
This study demonstrates that ER are present in osteogenic cells, and suggests that estrogen directly acts on medullary bone
osteogenesis. |
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Keywords: | Estrogen receptors Medullary bone Osteogenic cells Alkaline phosphatase Quail |
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