枳实及其提取物质量评价法的研究

 目的建立枳实及其提取物中辛弗林等生物碱类成分和橙皮苷等4种黄酮类成分的综合质量评价法。方法采用高效液相色谱法,以InertsilODS-3(4.6mm×250mm,5μm)色谱柱分别对枳实及其提取物中辛弗林生物碱类成分和新橙皮苷、橙皮苷、柚皮苷、芸香柚皮苷黄酮类成分进行测定。辛弗林:流动相为甲醇-水(7:4)(水中含0.02%磷酸和0.1%十二烷基硫酸钠),检测波长275nm,流速0.6mL·min^-1;橙皮苷等4种黄酮类成分:流动相为甲醇-水-冰醋酸(38:58:4),检测波长284nm,流速1.0mL·min^-1。结果辛弗林、橙皮苷、柚皮苷分别在0.04～0.64,0.03～0.48和0.03～0.48g·L^-1内,线性关系良好。加样回收率分别为98.3%,98.7%,97.9%。结论本方法准确,重现性好,可作为枳实及其提取物综合质量评价分析法。

Quality evaluation of raw materials and extract of Citrus aurantium

OBJECTIVE To establish a systematic quality evaluation for the raw materials and the extract of Citrus auran-tium.METHODS The contents of synephrine,neohesperidin,hesperidin, naringin and narirutin in the raw materials and the extracts of Citrus aurantium were determined by reversed-phase high performance liquid chromatography with an Inertsil ODS-3(4.6 mm×250 mm,5 μm) column, respectively. The chromatographic conditions for synephrine included the mobile phase consisting of methanol-water (composed of 0.02% phosphoric acid and 0.1 % sodium dodecyl sulfate) (7:4) at the flow rate of 0.6 mL·min^-1, and the detection wavelength at 275 nm. For the determination of 4 flavonone glycosides, a mixture of methanol, water and acetic acid (38:58:4) were used as the mobile phase at the flow rate of 1.0 mL·min^-1 ,with the detection wavelength at 284 nm.RESULTS The linear ranges were from 0.04 to 0.64 g·L^-1 for synephrine, from 0.03 to 0.48 g·L^-1 for hesperidin, and from 0.03 to 0.48 g·L^-1 for naringin. The recoveries were 98.3% , 98.7% and 97.9% , respectively. CONCLUSION The methods are accurate, reliable, and helpful for the systematic quality evaluation of the raw materials and the extract of Citrus aurantium.