bFGF和TGF-β1对原代培养的前列腺间质细胞的作用

目的:探讨碱性成纤维细胞生长因子(bFGF)和转化生长因子β1(TGF-β1)在良性前列腺增生(BPH)中的作用。方法:培养了人BPH间质细胞,采用MTT法检测无血清培养的间质细胞的增殖,用免疫组化方法检测平滑肌细胞表型变化,观察不同浓度bFGF和TGF-β1对培养的人BPH间质细胞的影响。结果:bFGF促进间质细胞增殖(P<0.05、P<0.01),较高浓度时(10μg/L)降低平滑肌细胞表型表达;TGF-β1(>0.1μg/L)抑制间质细胞增殖并增加平滑肌细胞表型表达(P<0.05、P<0.01);5μg/L的bFGF与0.001μg/L和0.01μg/L TGF-β1作用间质细胞,促进细胞增殖(P<0.01),与0.1μg/L,1μg/L及10μg/L TGF-β1作用间质细胞,抑制细胞增殖,0.1μg/L时对细胞的抑制作用轻微(P>0.05),1μg/L及10μg/L时出现明显的抑制(P<0.01),同时TGF-β1在较高浓度时(>1μg/L),平滑肌细胞表型表达明显增加(P<0.01)。结论:bFGF以时间和浓度依赖的方式促进培养的增生前列腺间质细胞的增殖,并减少平滑肌细胞表型表达;TGF-β1抑制间质细胞的生长并诱导间质细胞向平滑肌细胞分化,两者共同在BPH的形成机制中发挥着重要作用。

Role of bFGF and TGF-β1 in Primaritity Cultured Prostatic Stromal Cells

OBJECTIVE: To study the role of the basic fibroblast growth factor (bFGF) and transforming growth factor beta1 (TGF-beta1) in benign prostatic hyperplasia (BPH). METHODS: The human stromal cells of BPH were isolated and cultured. The proliferation of the stromal cells cultured in serum-free medium was detected by MTT method, the phenotype changes of smooth muscle cells detected by immunohistochemical method, and the effect of different concentrations of bFGF and TGF-beta1 on the cultured stromal cells of BPH observed. RESULTS: bFGF stimulated the cultured BPH stromal cell proliferation (P < 0.05, P < 0.01) and decreased the expression of smooth muscle cell (SMC) phenotype in higher concentration (10 microg/L). TGF-beta1 (> 1 microg/L) inhibited stromal cell proliferation and increased the expression of SMC phenotype (P < 0.05, P < 0.01). 5 microg/ml bFGF and TGF-beta1 (0.001 microg/L, 0.01 microg/L) promoted stromal cell proliferation (P < 0.01), while 5 microg/L bFGF and TGF-beta1 (0.1 microg/L, 1 microg/L, 10 microg/L) inhibited it, slightly in 0.1 microg/L (P > 0.05) and significantly in 1 microg/L and 10 microg/L (P < 0.01), and increased the expression of SMC phenotype in higher concentration (> 1 microg/L, P < 0.01). CONCLUSION: bFGF stimulates the proliferation of the prostatic stromal cells of BPH in a time- and dose-dependent fashion and decreases the expression of SMC phenotype, TGF-beta1 inhibits the growth of stromal cells and induces the differentiation of stromal cells to SMC, both playing an important role in the mechanism of BPH.